SCANNING CYTOMETRY FOR BONE MARROW PURGING

用于骨髓净化的扫描细胞术

• 批准号: 2767673
• 负责人: MANFRED R KOLLER
• 金额: $ 12.69万
• 依托单位: CYNTELLECT, INC.
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-15 至 2001-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2767673
• 关键词: biomedical equipment development; bone marrow purging; cell sorting; cell transplantation; flow cytometry; fluorimetry; hematopoietic stem cells; neoplastic cell; tissue /cell preparation

The purpose of this Phase I project is to develop a prototype scanning cytometry Photosis instrument and demonstrate its feasibility for eliminating tumor cells from hematopoietic stem cell (HSC) transplants. The two main components of this system, scanning cytometry and cell-by- cell laser ablation, have been previously demonstrated. The most novel aspect of this preliminary' work is the ability to perform rapid slide scanning while retaining image sharpness with a high performance autofocus and scanning system. The key, components of scanning cytometry - autofocus, image segmentation., image fluorometry and a 3 Hz field scanning rate - will be combined with laser ablation to create a prototype instrument. The scanning and laser ablation components will be combined into a single system and tested on a model system of tumor cell lines. This instrument will be fully automated: the operator will be required only to place the cells on the microscope stage and Photosis purification of l million cells over a 5 cm2 area (about l 0.000 fields) will occur in under an hour without further interaction with the operator. PROPOSED COMMERCIAL APPLICATION: The growth of autologous hematopoietic stern cell transplants is limited by the presence of contaminating tumor cells. No tumor cell purging procedures are in routine use Such procedures are needed, and their commercial potential is quite significant.

该项目的第一阶段的目的是开发一种原型扫描细胞仪Photosis仪器，并证明其消除造血干细胞（HSC）移植物中肿瘤细胞的可行性。该系统的两个主要组成部分，扫描细胞仪和细胞激光消融，以前已经证明。这项初步工作最新颖的方面是能够执行快速幻灯片扫描，同时保持高性能自动对焦和扫描系统的图像清晰度。扫描细胞仪的关键部件-自动对焦、图像分割。图像荧光测定法和3 Hz场扫描速率-将与激光烧蚀相结合，以创建原型仪器。扫描和激光消融组件将组合成一个系统，并在肿瘤细胞系的模型系统上进行测试。该仪器将是完全自动化的：操作员只需要将细胞放在显微镜载物台上，在5 cm 2面积（约10.000个视野）上的100万个细胞的光合作用纯化将在一小时内发生，而无需与操作员进一步互动。拟定商业应用：自体造血干细胞移植的生长受到污染肿瘤细胞的存在的限制。 没有常规使用的肿瘤细胞清除程序，这些程序是需要的，并且它们的商业潜力是相当显著的。