CALCIUM GRADIENTS AND EXOCYTOSIS IN CHROMAFFIN CELLS

嗜铬细胞中的钙梯度和胞吐作用

• 批准号: 6181262
• 负责人: JONATHAN R MONCK
• 金额: $ 10.61万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6181262
• 关键词: calcium channel; calcium flux; catecholamines; chromaffin cells; confocal scanning microscopy; cytoskeleton; exocytosis; guanine nucleotide binding protein; membrane fusion; tissue /cell culture; vesicle /vacuole; voltage /patch clamp

DESCRIPTION (adapted from the investigator's application): Ca2+ entry through voltage sensitive Ca2+ channels is the trigger for exocytosis in excitable cells. Recent evidence suggests that spatially localized Ca2+ gradients are important for regulating exocytosis. The goal of this proposal is to combine the pulsed laser Ca2+ imaging and confocal spot detection of Ca2+ transients with state-of-the-art methods for measuring exocytosis from single cells (patch clamp and voltametric measurements) in order to investigate the regulation of exocytosis by Ca2+ gradients in adrenal chromaffin cells. The experiments are designed to address the following questions. (1) What are the physiological mechanisms that control the development and extent of Ca2+ gradients at exocytotic sites? (2) What is the relationship between the Ca2+ gradients and exocytosis during depolarizing stimuli? (3) How do trains of short depolarizations, which are highly effective stimuli for exocytosis, alter the Ca2+ gradients and recruit secretory vesicles? (4) What are the roles of Ca2+ channel localization and Ca2+ buffers in Ca2+ signal localization? (5) Do the exocytotic sites behave as functional 'active zones'? And (6) what are the roles of Ca2+ gradients and GTP-binding proteins in recruitment of secretory vesicles to the exocytotic sites?

描述（改编自研究者的申请）：Ca2+进入

项目成果

Spatial distribution of Ca(2+) signals during repetitive depolarizing stimuli in adrenal chromaffin cells.

肾上腺嗜铬细胞重复去极化刺激期间 Ca(2) 信号的空间分布。

• DOI: 10.1016/s0006-3495(03)74759-6
• 发表时间: 2003
• 期刊: Biophysical journal.
• 作者: Marengo,FernandoD;Monck,JonathanR
• 通讯作者: Monck,JonathanR

Calcium gradients and exocytosis in bovine adrenal chromaffin cells.

• DOI: 10.1016/j.ceca.2005.06.006
• 发表时间: 2005-08
• 期刊: Cell calcium
• 影响因子: 4
• 作者: F. Marengo