ANALYSIS OF NEURAL DEVELOPMENT GENES IN DROSOPHILA

该项目的目标是识别和表征 都与果蝇的神经发育有关。一种遗传修饰物 筛选与器官类型选择器基因切割相互作用的基因 结果至少鉴定了13个基因，这些基因 似乎在神经发生中起作用。其中四个是以前的 有特征的基因和其余的是未特征的致死P元件 插入突变体。这些突变基因中的大多数都表现出某种类型的 神经细胞特异性抗体染色时出现神经元缺陷。这项建议 旨在关注屏幕上发现的三个最有希望的基因。 这些基因的突变表型将进一步用 细胞型特异性抗体电池。然后将使用以下方法克隆这些基因 用质粒拯救技术回收相应的片段 基因组DNA。从这一点来看，标准文库筛选和测序 技术将被用来完成克隆项目。表达方式 然后将通过原位杂交来分析这些基因的模式。这个 过表达表型将使用UAS-GAL4系统进行表征。 这些基因还将在与其他基因的遗传上位性实验中进行测试 已知的神经发育基因来确定这些 基因位于导致神经发育的遗传层次中。

The goal of the project is to identify and characterize genes that are involved in the neural development of Drosophila. A genetic modifier screen for genes that interact with the organ-type selector gene cut was carried out, resulting in the identification of at least thirteen genes that appear to play a role in neurogenesis. Four of these are previously characterized genes and the remainder are uncharacterized lethal P-element insertion mutants. A majority of these mutant genes exhibit some type of neuronal defect when stained with neural-specific antibodies. This proposal aims to focus on three of the most promising genes identified in the screen. The mutant phenotype of these genes will be further characterized using a battery of cell-type specific antibodies. The genes will then be cloned using the plasmid rescue technique to recover a fragment of the corresponding genomic DNA. From that point, standard library screening and sequencing techniques will be used to complete the cloning project. The expression patterns of the genes will then be analyzed by in situ hybridization. The overexpression phenotype will be characterized using the UAS-GAL4 system. These gene will also be tested in genetic epistasis experiments with other known neural development genes to ascertain the precise positions that these genes occupy within the genetic hierarchy leading to neural development.