RELATIONSHIP BETWEEN TROPISM, INFECTIVITY, AND NEUTRALIZATION IN HIV

HIV 的趋向性、感染性和中和作用之间的关系

• 批准号: 6161248
• 负责人: K PEDEN
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6161248
• 关键词: CD4 molecule; HIV envelope protein gp120; HIV envelope protein gp41; gene mutation; genetic regulatory element; genetic strain; human immunodeficiency virus 1; human immunodeficiency virus 2; tissue /cell culture; transfection; virus RNA; virus genetics; virus infection mechanism; virus replication

We are trying to understand why certain HIV isolates enter and infect some cell types but not others. For example, some HIV isolates are able to infect primary T cells, while others are able to infect both primary T cells and macrophages; some isolates have the capacity to induce the formation of large multinuclear cells (syncytia) while others cannot. Furthermore, we have found that certain viruses can alter their biological characteristics by passage in tissue culture, and the genetic changes that permit this to occur are in the HIV envelope, the protein involved in the initial binding and uptake of the virus. Thus, part of the understanding of HIV tropism involves understanding the biochemical events involved in virus binding and uptake. Because binding and entry is not the whole story of viral tropism, we are investigating determinants of tropism in other regions of the viral genome. Our work involves using assays to measure virus growth, virus amounts, and virus neutralization. A familiarity with these assays and methods is important to the FDA review process, where such assays are used to ascertain the efficacy of vaccines against HIV.

我们正试图了解为什么某些艾滋病毒分离株进入并感染一些人， 细胞类型，而不是其他类型。 例如，一些HIV分离株能够 感染原代T细胞，而另一些则能够同时感染原代T细胞 细胞和巨噬细胞;一些分离株有能力诱导 形成大的多核细胞（合胞体），而其他细胞则不能。 此外，我们发现某些病毒可以改变它们的生物学特性， 通过在组织培养中传代， 允许这种情况发生的是在HIV包膜中， 病毒的初始结合和吸收。 因此， 艾滋病病毒嗜性的研究涉及了解 病毒结合和摄取。 因为捆绑和进入并不是全部 关于病毒嗜性的故事，我们正在研究 病毒基因组的其他区域。 我们的工作包括使用检测方法来测量病毒生长、病毒数量， 病毒中和 熟悉这些测定和方法是 重要的FDA审查过程中，这些测定用于 确定艾滋病毒疫苗的有效性。