BUNYAVIRUS/MOSQUITO INTERACTIONS
BUNYAVIRUS/MOSQUITO INTERACTIONS
批准号:
6196473
负责人:
Thomas M Yuill
金额:
$21.27万
依托单位国家:
美国
项目类别:
财政年份:
1995
资助国家:
美国
项目状态:
已结题
起止时间:
1995-04-01 至 2004-06-30
关键词:
Aedes Bunyaviridae complementary DNA disease /disorder model disease vectors encephalitis virus gene mutation glycoproteins host organism interaction laboratory mouse molecular cloning nervous system infection tissue /cell culture virulence virus diseases virus infection mechanism virus protein virus replication
中文摘要
描述(改编自申请人的摘要):本发明的总体目标是:
研究的目的是检查最初的分子相互作用,
确定布尼亚病毒感染蚊子能力的第一步
vector.布尼亚病毒是人类疾病的重要原因,
世界各地的家畜大多数布尼亚病毒传播给人类,
动物蚊子他们将使用拉克罗斯脑炎病毒(LACV),
其自然蚊子媒介三列伊蚊用于这些研究。他们将
研究病毒糖蛋白在病毒与蚊子结合中的作用
中肠,在感染Ae.三列的LACV,并在特异性
选定蚊子物种的LACV。它们将完全产生传染性LACV
使用基于RNA聚合酶1的系统,
逆转流感病毒。它们会引入特定的突变
以及嵌合片段和基因到LACV中。基于大量的工作
在他们的实验室中,他们假设拉克罗斯的G2包膜蛋白
病毒对于病毒与蚊子中肠细胞的结合至关重要,
因此对蚊子感染至关重要。他们将确定蛋白质序列
的G2,这是需要的结合拉克罗斯病毒的中肠细胞,
它的自然蚊子媒介,以及另一种允许的蚊子物种。他们
将确定载体特异性是否部分由肽介导
病毒糖蛋白中的序列,如果这些序列相同,
测定病毒与感受态载体蚊子中肠细胞的结合。的
开发有效的技术来产生含有
特异性突变将允许在体内研究单个基因
在蚊子感染和哺乳动物发病机制中的作用
英文摘要
DESCRIPTION (Adapted from the Applicant's Abstract): The overall goal of this
research is to examine the initial molecular interactions that are a critical
first step in determining the ability of a bunyavirus to infect ist mosquito
vector. Bunyaviruses are a significant cause of disease in humans and in
domestic animals worldwide. Most bunyaviruses are transmitted to humans and
animals by mosquitoes. They will use La Crosse encephalitis virus (LACV) and
its natural mosquito vector Aedes triseriatus for these studies. They will
examine the role viral glycoproteins play in the binding of virus to mosquito
midgut, in the infection of Ae. triseriatus by LACV, and in the specificity of
LACV for selected mosquito species. They will generate infectious LACV entirely
from cloned cDNA using a RNA polymerase 1-based system that was recently used
to reverse engineer influenza viruses. They will introduce specific mutatuions
and chimeric segments and genes into LACV. Based on a significant body of work
in their laboratory, they hypothesize that the G2 envelope protein of La Crosse
virus is critical for virus binding to the cells of the mosquito midgut, and
thus critical for mosquito infection. They will determine the protein sequences
of G2 that are required for biding of La Crosse virus to the midgut cells of
its natural mosquito vector, and to another permissive mosquito species. They
will determine if vector specificity, in part, is mediated by pepetide
sequences in the viral glycoproteins and if these sequences are the same that
determine virus binding to midgut cells of competent vector mosquitoes. The
development of efficient techniques to generate bunyaviruses containing
specific mutations will permit in vivo investigations of individual gene
functions in mosquito infections and in mammalian pathogenesis
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会议论文
BUNYAVIRUS/MOSQUITO INTERACTIONS
-
批准号:6651575
-
项目类别:
-
资助金额:$21.6万
-
财政年份:1995
-
负责人:Thomas M Yuill
-
依托单位:
BUNYAVIRUS/MOSQUITO INTERACTIONS
-
批准号:6373407
-
项目类别:
-
资助金额:$21.6万
-
财政年份:1995
-
负责人:Thomas M Yuill
-
依托单位:
BUNYAVIRUS/MOSQUITO INTERACTIONS
-
批准号:6534050
-
项目类别:
-
资助金额:$19.44万
-
财政年份:1995
-
负责人:Thomas M Yuill
-
依托单位:
BUNYAVIRUS/MOSQUITO INTERACTIONS
-
批准号:2390407
-
项目类别:
-
资助金额:$15.75万
-
财政年份:1995
-
负责人:Thomas M Yuill
-
依托单位:
BUNYAVIRUS/MOSQUITO INTERACTIONS
-
批准号:2072635
-
项目类别:
-
资助金额:$15.14万
-
财政年份:1995
-
负责人:Thomas M Yuill
-
依托单位:
SMALL INSTRUMENTATION GRANT
-
批准号:3525195
-
项目类别:
-
资助金额:$1.96万
-
财政年份:1991
-
负责人:Thomas M Yuill
-
依托单位:
SMALL INSTRUMENTATION PROGRAM
-
批准号:3525165
-
项目类别:
-
资助金额:$1.19万
-
财政年份:1990
-
负责人:Thomas M Yuill
-
依托单位:
MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM
-
批准号:3512752
-
项目类别:
-
资助金额:$5.0万
-
财政年份:1987
-
负责人:Thomas M Yuill
-
依托单位:
CRITICAL POINT DRYER; LOW TEMPERATURE SECTION SYSTEM
-
批准号:3526073
-
项目类别:
-
资助金额:$1.21万
-
财政年份:1987
-
负责人:Thomas M Yuill
-
依托单位:
MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM
-
批准号:3512749
-
项目类别:
-
资助金额:$0.45万
-
财政年份:1987
-
负责人:Thomas M Yuill
-
依托单位:
MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM
-
批准号:3512747
-
项目类别:
-
资助金额:$0.45万
-
财政年份:1987
-
负责人:Thomas M Yuill
-
依托单位:
MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM
-
批准号:3512750
-
项目类别:
-
资助金额:$1.2万
-
财政年份:1987
-
负责人:Thomas M Yuill
-
依托单位:
MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM
-
批准号:3512751
-
项目类别:
-
资助金额:$5.0万
-
财政年份:1987
-
负责人:Thomas M Yuill
-
依托单位:
MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM
-
批准号:3512748
-
项目类别:
-
资助金额:$0.45万
-
财政年份:1987
-
负责人:Thomas M Yuill
-
依托单位:
BIOMEDICAL RESEARCH SUPPORT GRANT
-
批准号:3517158
-
项目类别:
-
资助金额:$4.91万
-
财政年份:1987
-
负责人:Thomas M Yuill
-
依托单位:
BIOMEDICAL RESEARCH SUPPORT GRANT
-
批准号:3517157
-
项目类别:
-
资助金额:$4.35万
-
财政年份:1986
-
负责人:Thomas M Yuill
-
依托单位:
BIOMEDICAL RESEARCH SUPPORT GRANT
-
批准号:3517156
-
项目类别:
-
资助金额:$3.47万
-
财政年份:1985
-
负责人:Thomas M Yuill
-
依托单位:
BIOMEDICAL RESEARCH SUPPORT GRANT
-
批准号:3517161
-
项目类别:
-
资助金额:$5.23万
-
财政年份:1984
-
负责人:Thomas M Yuill
-
依托单位:
BIOMEDICAL RESEARCH SUPPORT GRANT
-
批准号:3517160
-
项目类别:
-
资助金额:$5.54万
-
财政年份:1984
-
负责人:Thomas M Yuill
-
依托单位:
BIOMEDICAL RESEARCH SUPPORT GRANT
-
批准号:3517159
-
项目类别:
-
资助金额:$5.21万
-
财政年份:1984
-
负责人:Thomas M Yuill
-
依托单位:
海外基金