GIARDIA--A MODEL FOR ANCIENT EUKARYOTE GENOME FUNCTIONS

贾第鞭毛虫——古代真核生物基因组功能的模型

• 批准号: 6170750
• 负责人: FRANCES D. GILLIN
• 金额: $ 26.28万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6170750
• 关键词: Giardia; animal genetic material tag; biochemical evolution; enzyme activity; eukaryote; gene expression; genetic transcription; genome; isomerase; mitochondria; molecular genetics; nucleic acid sequence; polymerase chain reaction; prokaryote; protein biosynthesis; protein folding; protein transport

As a model for functional-biological genome analyses, Giardia provides unique perspectives on the origins of eukaryotic cells. This proposal belongs to an Interactive Research Project Grant (IRPG), which will synergize strongly with an R01 "Giardia: A Model for Ancient Eukaryote Genome Analysis" (Mitchell Sogin PI, projected start date Feb. 1. 1998), whose overall goal is to obtain accurate sequence and mapping information for at least 95 percent of this small, but evolutionarily crucial genome. Our tests of inferred function of a carefully selected sample will greatly extend and validate the sequence information. The major goal unifying these biological analyses is to obtain insights into the genetic innovations that led to compartmentalization of important functions in eukaryotic cells with the following Specific Aims: The view that Giardia is "pre-mitochondrial" has been challenged by our finding of three mitochondrial-like genes whose expression, location and activities we will analyze in Specific Aim 1. In Specific Aim 2, we will gain insights into the evolution of eukaryotic endomembrane systems by analyses of three giardial protein disulfide isomerases that have both pro- and eukaryotic properties. Since the organization of genetic material is increasingly complex in eukaryotes, in Specific Aim 3, we will analyze a model region of very high transcript density to elucidate strategies that Giardia can use to efficiently exploit its limited genome size. In Specific Aim 4, we request the capability to analyze novel genes chosen for their power to extend our understanding of the complexity of eukaryotic cellular compartments or of giardial pathogenesis. This project will provide novel insights into the evolution of basic eukaryotic cellular mechanisms, as well as specific information on an important environmentally transmitted infectious agent. It has the unique potential to reveal biological innovations reflective of the prokaryotic/eukaryotic divergence.

作为功能生物基因组分析的模型，贾第虫提供了 关于真核细胞起源的独特观点。这项建议 属于互动研究项目补助金（IRPG），将 与R 01“贾第虫：古真核生物模型”有很强协同作用 基因组分析”（米切尔Sogin PI，预计开始日期2月1日。1998年）， 其总体目标是获得准确的序列和映射 至少95%的这种小的，但进化的信息， 关键基因组 我们对精心挑选的 样本将极大地扩展和验证序列信息。的 统一这些生物学分析的主要目标是深入了解 基因创新导致了重要基因的划分， 在真核细胞中的功能，具有以下特定目的： 贾第虫是“前线粒体”的说法受到了我们发现的挑战 三个类神经胶质瘤基因的表达，位置和 我们将在具体目标1中分析这些活动。 在具体目标2中， 将深入了解真核生物内膜系统的进化 通过分析三种具有 既有原核生物的特性也有真核生物的特性 由于遗传学的组织 材料在真核生物中越来越复杂，在具体目标3中，我们 将分析一个非常高的转录本密度的模型区域，以阐明 贾第虫可以有效利用其有限的 基因组大小 在具体目标4中，我们要求具备分析能力 选择新的基因是因为它们的力量可以扩展我们对 真核细胞区室或心血管的复杂性 发病机制 该项目将提供新的见解， 进化的基本真核细胞机制，以及具体的 关于一种重要的环境传播传染病的资料 剂 它具有揭示生物学创新的独特潜力 反映了原核/真核的分歧。