HIV 1 STRAIN DIVERSITY AND NONPROGRESSORS

HIV 1 毒株多样性和非进展者

• 批准号: 6169953
• 负责人: HAYNES W SHEPPARD
• 金额: $ 23.06万
• 依托单位: PUBLIC HEALTH FOUNDATION ENTERPRISES
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6169953
• 关键词: AIDS; CD antigens; CD4 molecule; HIV infections; SCID mouse; apoptosis; clinical research; flow cytometry; genetic strain; human immunodeficiency virus 1; human subject; leukocyte activation /transformation; long term survivor; longitudinal human study; male; pathologic process; phenotype; virulence; virus RNA; virus load

DESCRIPTION: (Adapted from investigator's abstract) The main purpose of this proposal is to define the variability in virulence among HIV-1 isolates from individuals with widely divergent progression rates and to assess the interrelationships between virus burden, phenotype, and the capacity to induce immune activation/apoptosis, in a SCID-hu mouse model. AIM 1 is to continue to determine the relationship between virus burden, markers of immune activation/apoptosis and CD4 decline in longitudinal studies of highly characterized LTNP and RP from the SFMHS. Parallel studies of quasispecies diversity, the in vitro phenotype of virus isolates, and various immune responses are being conducted with these subjects under other funding. AIM 2 is to further assess the importance of viral diversity by characterizing the phenotypic behavior of HIV-1 isolates from LTNP and NP (10 each) in an improved SCID mouse model. Measurements to be made in mice include: 1) thymic and/or peripheral CD4+ cell loss 2) plasma viral RNA and 3) level of various markers of immune activation and apoptosis.

描述：（改编自研究者摘要） 该建议旨在确定HIV-1分离株之间毒力的变异性 从进展率差异很大的个体中， 病毒负荷，表型和能力之间的相互关系， 在SCID-hu小鼠模型中诱导免疫活化/凋亡。 目的1是继续确定病毒负荷， 免疫活化/凋亡和CD 4下降的标志物 从SFMHS高度表征的LTNP和RP的研究。 平行 准种多样性的研究，病毒分离株的体外表型， 这些受试者的各种免疫反应 其他资金。 目的2是进一步评估病毒多样性的重要性 通过表征来自LTNP和NP的HIV-1分离株的表型行为， (10每个）在改进的SCID小鼠模型中。 在小鼠中进行测量 包括：1）胸腺和/或外周CD 4+细胞损失，2）血浆病毒RNA， 3)免疫激活和细胞凋亡的各种标志物的水平。