REGULATION OF THE MURINE CALCITONIN RECEPTOR GENE
鼠降钙素受体基因的调控
基本信息
- 批准号:6171136
- 负责人:
- 金额:$ 33.69万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1998
- 资助国家:美国
- 起止时间:1998-08-01 至 2002-07-31
- 项目状态:已结题
- 来源:
- 关键词:DNA binding protein RNase protection assay calcitonin cell differentiation dexamethasone embryonic stem cell gene targeting genetic regulation genetic regulatory element genetically modified animals hormone receptor interleukin 1 laboratory mouse molecular cloning osteoclasts polymerase chain reaction receptor expression transcription factor
项目摘要
DESCRIPTION (adapted from the Investigator's abstract): Calcitonin (CT)
was originally identified as an endocrine hormone that lowers
extracellular calcium by inhibiting osteoclastic bone resorption and
enhancing renal calcium excretion. The identification of CT receptors in
diverse tissues and cell types and the demonstration of its effects on the
developing embryo indicates that CT may serve a more complex and diverse
function than its role in regulating homeostasis. The discovery that the
calcitonin receptor (CTR) gene is alternatively spliced and that certain
CTR isoforms may bind ligands other than CTL provides further insight into
the molecular and biochemical basis for this functional diversity. In the
proposed studies, the Principal Investigators will 1) Clone and
characterize the murine CTR gene and identify the transcription start
site(s) in osteoclasts and other mCTR-expressing cell-types. 2) Define the
specific and potentially unique regulatory sequences responsible for
expression of mCTR expressing cells and tissues and identify the putative
transacting transcription factors that bind to these sequences. A variety
of experimental approaches will be used to define the factors controlling
mCTR expression in osteoclasts and other cell types during development.
This will include defining the sequences involved in the regulation of
mCTR gene expression by calcitonin (CT) and other hormones (e.g.,
dexamethasone) or cytokines (e.g., IL-1). It is anticipated that the
regulatory factors that control mCTR gene expression in osteoclasts also
are involved in the regulation of the expression of other genes that
accompany the final stages of osteoclast differentiation and activation.
3) Investigate the developmental regulation of the mCTR gene. The temporal
and cell-specific pattern of mCTR gene expression will be documented, and
then transgenic mCTR-lacZ reporter mice will be used to analyzed the
relevance of the regulatory regions identified in Aim 2 and to define any
other sequences that may be important for developmentally correct
expression in vivo. 4) Determine the effect of targeted disruption of the
mCTR gene on osteoclasts and other mCTR-expression tissues, and on mouse
development. The Principal Investigator and her colleagues and her
colleagues will generate transgenic mice with a targeted disruption of the
mCTR gene. In addition, they will develop ES cell differentiation
techniques to generate osteoclasts from E cells for studying the
regulation of mCTR gene expression during osteoclast differentiation and
development.
描述(改编自研究者摘要):降钙素(CT)
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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DEBORAH Lynn GALSON其他文献
DEBORAH Lynn GALSON的其他文献
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{{ truncateString('DEBORAH Lynn GALSON', 18)}}的其他基金
GFI-1 and Osteoblast Suppression in Multiple Myeloma
多发性骨髓瘤中的 GFI-1 和成骨细胞抑制
- 批准号:
8290562 - 财政年份:2010
- 资助金额:
$ 33.69万 - 项目类别:
GFI-1 and Osteoblast Suppression in Multiple Myeloma
多发性骨髓瘤中的 GFI-1 和成骨细胞抑制
- 批准号:
8117780 - 财政年份:2010
- 资助金额:
$ 33.69万 - 项目类别:
GFI-1 and Osteoblast Suppression in Multiple Myeloma
多发性骨髓瘤中的 GFI-1 和成骨细胞抑制
- 批准号:
8681152 - 财政年份:2010
- 资助金额:
$ 33.69万 - 项目类别:
GFI-1 and Osteoblast Suppression in Multiple Myeloma
多发性骨髓瘤中的 GFI-1 和成骨细胞抑制
- 批准号:
7942687 - 财政年份:2010
- 资助金额:
$ 33.69万 - 项目类别:
GFI-1 and Osteoblast Suppression in Multiple Myeloma
多发性骨髓瘤中的 GFI-1 和成骨细胞抑制
- 批准号:
8490313 - 财政年份:2010
- 资助金额:
$ 33.69万 - 项目类别:
Viral and Genetic Regulation of Abnormal OCL Activity in PD
PD 中异常 OCL 活性的病毒和遗传调控
- 批准号:
8123401 - 财政年份:2008
- 资助金额:
$ 33.69万 - 项目类别:
Viral and Genetic Regulation of Abnormal OCL Activity in PD
PD 中异常 OCL 活性的病毒和遗传调控
- 批准号:
7680931 - 财政年份:2008
- 资助金额:
$ 33.69万 - 项目类别:
Viral and Genetic Regulation of Abnormal OCL Activity in PD
PD 中异常 OCL 活性的病毒和遗传调控
- 批准号:
7901075 - 财政年份:2008
- 资助金额:
$ 33.69万 - 项目类别:
Viral and Genetic Regulation of Abnormal OCL Activity in PD
PD 中异常 OCL 活性的病毒和遗传调控
- 批准号:
7689304 - 财政年份:2008
- 资助金额:
$ 33.69万 - 项目类别:
Viral and Genetic Regulation of Abnormal OCL Activity in PD
PD 中异常 OCL 活性的病毒和遗传调控
- 批准号:
8315745 - 财政年份:2008
- 资助金额:
$ 33.69万 - 项目类别:
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