COORDINATED CONTROL OF HPV TRANSCRIPTION AND REPLICATION

描述：(改编自调查人员摘要)：在本修订的新 来自一名年轻的独立调查员的申请，一系列生物物理 人们建议进行研究，以探讨这一假说背后的机制 乳头瘤病毒转录与DNA的协同调控 复制。私家侦探亚历山大博士假设这个坐标 调控是细胞转录和细胞间竞争的结果。 复制因子和病毒E1和E2蛋白调节和 有助于转录和复制。这项提议的目的是 评估这些因素对彼此DNA结合、蛋白质的影响 结合活性和酶活性。从这项研究中获得的数据将用于 要创建HPV转录和复制控制的动态模型， 将广泛应用于DNA的转录和复制控制 病毒。

DESCRIPTION: (Adapted from the Investigator's abstract): In this revised new application from a young independent investigator, a series of biophysical studies are proposed to investigate the mechanisms underlying the hypothesized coordinate regulation of papillomaviral transcription and papillomaviral DNA replication. The PI, Dr. Alexander, hypothesizes that this coordinate regulation is a consequence of competition between cellular transcription and replication factors and the viral E1 and E2 protein that regulate and contribute to transcription and replication. The aims of this proposal are to evaluate the influence of these factors on each other's DNA binding, protein binding and enzymatic activities. The data gained from this study will be used to create a dynamic model of HPV transcription and replication control that will be broadly applicable to transcription and replication control in DNA viruses.