A NOVEL APOPTOSIS ASSAY WITH ANTIBODIES TO SSDNA

描述(改编自申请人的摘要)：发展 需要灵敏、特异和通用的分析来确定其作用 生物学中的细胞凋亡和肿瘤细胞对化疗的反应。一本小说 申请人开发的抗单链DNA单抗检测就是为满足这些要求而设计的 要求。该检测是基于一种新的对凋亡细胞的染色 针对单链DNA的高度特异性的单抗。重要的是 单抗检测组织切片中的细胞凋亡，对于这些组织切片，唯一的其他 现有的组织化学标记物(TUNEL)敏感性低、局限性大 专一性。这项提议的主要目的是验证一部小说 抗单链DNA单抗与其他可用检测的对照试验：Annexin V Caspase染色、激活、线粒体膜电位降低 DNA片段化(TUNEL染色和DNA梯状条带)。修改后的协议， 这使得这项技术在档案和活检中的应用成为可能 材料，将被开发出来。该检测将在#年在不同的模型上进行测试。 命令证明普遍适用并确定与药物的相关性 敏感性：细胞毒剂、激素、Fas结扎诱导的细胞凋亡 造血细胞和上皮细胞系中的受体、细胞凋亡和细胞凋亡 药物敏感和耐药细胞系和实体瘤。 建议的商业应用：用于市场上的细胞凋亡检测。

DESCRIPTION (Adapted from the applicant's abstract): The development of sensitive, specific and universal assay is needed to determine the role of apoptosis in biology and in tumor cell response to chemotherapy. A novel anti-ssDNA MAb assay developed by the applicant is designed to meet these requirements. The assay is based on the staining of apoptotic cells with a new monoclonal antibody (MAb) highly specific for single-stranded DNA. Importantly, MAb assay detects apoptosis in tissue sections, for which the only other available histochemical marker (TUNEL) has low sensitivity and limited specificity. The major goal of this proposal is validation of a novel anti-ssDNA MAb apoptosis assay against other available tests: annexin V staining, activation of caspases, decrease in mitochondria membrane potential and DNA fragmentation (TUNEL staining and DNA ladder). Modified protocols, which make possible application of the technique to archival and biopsy material, will be developed. The assay will be tested in different models in order to prove universal application and to determine correlation with drug sensitivity: apoptosis induced by cytotoxic agents, hormones, ligation of FAS receptor, apoptosis in hematopoetic and epithelial cell lines and apoptosis in drug-sensitive and drug resistant cell lines and solid tumors. PROPOSED COMMERCIAL APPLICATION: To market apoptosis assay.