TAFS AND GENERAL TRANSCRIPTION FACTORS IN YEAST

描述（改编自摘要）：II类基因表达的启动 在真核生物中涉及形成一个大的多亚基复合体 在启动子上含有RNA聚合酶II和其它因子。 TATA结合蛋白（TBP）在这一过程中起着核心作用，因为它结合 直接连接到启动子DNA上。 至少5种不同的TBP相关蛋白 已经鉴定出在II类基因中具有作用的复合物， 转录，但它们对基因表达调控的贡献 这些TBP结合蛋白包括TAFII、NC 2 （Dr1-DRAP 1）、MOT 1、NOT和SPT。 基因和生化实验 本提案中所述的目的是调查性质， TBP结合蛋白（TAFIIs除外）在II类控制中的功能 基因在S.啤酒。 该提案的四个具体目标 研究了RNA聚合酶II和RNA聚合酶II之间的遗传相互作用， 全酶和TBP结合蛋白在体内，2）调查的影响 TBP结合蛋白突变对II类基因表达的影响， 3）纯化和鉴定各种亚基组成， TBP结合蛋白参与S.酿酒酵母 利用生物化学方法，4）研究转录功能， TBP结合蛋白使用野生型和突变蛋白， 体外转录系统。

DESCRIPTION (adapted from abstract): Initiation of class II gene expression in eukaryotes involves the formation of a large multisubunit complex containing RNA polymerase II and additional factors on promoters. TATA-binding protein (TBP) plays a central role in this process as it binds directly to promoter DNA. At least five different TBP-associated protein complexes have been identified that have roles in class II gene transcription, but their contributions to the regulation of gene expression are not yet well understood These TBP-binding proteins include TAFIIs, NC2 (Dr1-DRAP1), MOT1, NOTs and SPTs. The genetic and biochemical experiments described in this proposal are designed to investigate the nature and function of TBP-binding proteins other than TAFIIs in control of class II gene expression in S. cerevisiae. The four specific aims of this proposal are: 1) to study the genetic interaction betwee RNA polymerase II holoenzyme and TBP-binding proteins in vivo, 2) to investigate the effects of mutations in the TBP-binding proteins on class II gene expression in vivo, 3) to purify and identify the subunit composition of the various TBP-binding proteins involved in class II gene expression in S. cerevisiae using biochemical approaches, 4) to study the transcriptional functions of the TBP-binding proteins using wild-type and mutant proteins in transcription systems in vitro.