GENE TRANSFER TO THE VASCULAR ENDOTHELIUM

描述（来自申请的逐字描述）：这些研究的目的是 使用噬菌体展示文库的体内淘选方法来鉴定归巢 肽的心脏新血管，冠状动脉内皮和 内分泌我们希望使用这些肽，特别是原型RGD 作为靶向逆转录病毒和HIV慢病毒配体的新生血管归巢肽 这些组织的载体。我们还将研究一种方法， 来自组合逆转录病毒文库的高效RGD靶向载体。 最后，我们建议描述这些靶向药物的生物分布特征。 通过灵敏的荧光方法检测载体，并将载体附着与 内皮特异性基因表达的位点。

DESCRIPTION (Verbatim from the application): The goal of these studies is to use in vivo panning methods with phage display libraries to identify homing peptide for the heart neovasculature, the coronary endothelium and the endocardium. We wish to use these peptides, especially the prototype RGD neovascular homing peptide, as ligands to target retrovirus and HIV lentivirus vectors to those tissues. We will also examine a method for selecting more efficient RGD-targeted vectors from a combinatorial retrovirus library. Finally, we propose to characterize the biodistribution of these targeted vectors by sensitive fluorescence methods and correlate vector attachment with sites of endothelial-specific gene expression.