TECHNOLOGY FOR AUTOMATED ANALYSIS OF PROTEOMES

生物学研究中最引人注目的发展之一是从单一基因和蛋白质的分析转向生物系统和途径的综合分析。这种转变是自动化、高通量基因组技术的发展及其在全基因组范围内测序和测量基因表达的应用的结果。目前，没有强大的技术可用于在蛋白质水平上分析生物系统。然而，蛋白质是最重要的一类生物效应分子，如果不知道所涉及的蛋白质的身份、功能和活性状态，就不能建立完整的生物过程模型。本申请中提出的工作旨在开发一种技术，用于快速、灵敏、定量和全面分析复杂生物样品中的蛋白质表达谱，最终分析全细胞裂解物。一旦发展起来，这种技术将成为生物学家试图从生物系统的功能、控制和机制方面解释基因组线性信息的重要工具。

One of the most dramatic current developments in biological research is the shift from the analysis of single genes and proteins to the comprehensive analysis of biological systems and pathways. This shift is a consequence of the development of automated, high throughput genomic technologies and their application to sequence complete genomes and to measure gene expression on a genome-wide scale. Currently, no comparably powerful technology is available for the analysis of biological systems on the protein level. Howe er, proteins are the most significant class of biological effector molecules, and a complete model of a biological process cannot be established without knowledge of the identify, function and state of activity of the proteins involved. The work proposed in this application is aimed at the development of a technology for the rapid, sensitive, quantitative and comprehensive analysis of protein expression profiles in complex biological samples, ultimately in whole cell lysates. Once developed such a technology will be an essential tool for biologists' attempts to interpret the linear information of genomes in terms of function, control and mechanisms of biological systems.