Core--Protein production

蛋白质生产核心旨在为生化研究提供大量纯化的蛋白质试剂。每个拟议的项目都在很大程度上依赖于展示和表征蛋白质之间的相互作用，评估纯化蛋白质的酶活性，并使用纯化蛋白质开发试剂(例如抗体)。因此，这一核心将通过为使用细菌和真核表达系统生产蛋白质提供集中和有效的设施，从而使方案项目受益。核心由两个部分组成，一个用于提供细菌表达的蛋白质，另一个用于提供使用杆状病毒系统表达的蛋白质。核心将配备一名合格的技术人员，负责开展核心的两个组成部分的活动。

The Protein Production Core is designed to provide large quantities of purified protein reagents for biochemical studies. Each of the proposed projects relies heavily on demonstrating and characterizing protein-protein interactions, assessing the enzymatic activities of purified protein and using purified proteins for reagent development (e.g., antibodies). Thus this Core will benefit the Program Project by providing a centralized and efficient facility for the production of proteins using bacterial and eucaryotic expression systems. The Core has two component parts, one designed to provide bacterially expressed proteins and the second to provide proteins expressed using the baculovirus system. The Core will be staffed with a qualified technician who will be responsible for carrying out the activities of both of the components of the Core.