ANALYSIS OF THE HDLG-DEPENDENT PROTEIN COMPLEX

描述：根据他们的工作和其他实验室的工作，这些 调查人员提出hDlg是一个组成部分，可能是必不可少的 质膜多蛋白质复合体的成核剂- 相关的信号分子。这一假设是基于 观察到hDlg序列与新的推定的APC、Abl、Crk、Lck的结合 称为PBK1(PDZ结合激酶1)的双特异性蛋白激酶， T/SXV基序通道蛋白、含SH3的蛋白、多聚脯氨酸- 含有蛋白质和/或蛋白质4.1 oezrin。拟议的研究 我将通过以下方式验证这一假设：(1)细化交互作用图 HDlg和4.1/ERM蛋白之间的结构域及其作用 靶向膜的相互作用；(2)定量和精制 对hdlg与自身关联的理解；(3)量化和 完善合作伙伴；以及(4)评估自贸区的影响- HDIg与一种可能的激酶之间的相互作用 一种酵母双杂交筛网。长期目标是更好地理解 该肿瘤的作用方式和生化特征 抑制蛋白。

DESCRIPTION: Based on their work and that of other laboratories, these investigators propose that hDlg is a component, possibly the essential nucleating agent, of a multi-protein complex of plasma membrane- associated signaling molecules. This hypothesis is based on the observed binding of hDlg sequences to APC, Abl, Crk, Lck, a new putative dual-specificity protein kinase called PBK1 (PDZ-Binding Kinase 1), T/SXV motif channel proteins, SH3-containing proteins, polyproline- containing proteins, and/or protein 4.1 oezrin. The proposed research will test this hypothesis by: (1) refining the map of interaction domains between hDlg and 4.1/ERM proteins and the role of this interaction in targeting to the membrane; (2) quantifying and refining the understanding of hDlg association with itself; (3) quantifying and refining the partners; and (4) assessing the implication of the PDZ- mediated association between hDlg and a putative kinase identified by a yeast two-hybrid screen. The long-term goal is to understand better the mode of action and biochemical characteristics of this tumor suppressor protein.