STRUCTURES AND FUNCTIONS OF CYS3HIS ZINC DOMAINS

描述：(改编自申请者的摘要)各种类别的 参与基因表达调控的蛋白质已被发现 包含形成小结构域的短伸展氨基酸 围绕结合的锌离子组织。这些蛋白质中的许多通过 与核酸的相互作用。当前感兴趣的两个类 是CCHC-box结构域蛋白和CCCH结构域蛋白。CCHC-BOX 结构域首次被鉴定为逆转录病毒蛋白质的一种成分 其中，这个领域似乎在单项识别中起着关键作用 搁浅的DNA。已经建立了这些相互作用的结构模型。 这将通过研究与之结合的蛋白质片段来测试 锥虫体内特异的单链DNA序列。CCCH域 在Nup475蛋白中被识别，其水平随着 胰岛素和其他生长因子。CCCH域的功能不是 已经确定了，但最近的结果表明，它们可能会切割特定的 RNA结构。这项提议反映了一种合作努力，以 用溶液状态核磁共振结构表征这两个结构基序 以及功能性和约束性研究。

DESCRIPTION: (adapted from applicant's abstract) A variety of classes of proteins involved in the regulation of gene expression have been found to contain short stretches of amino acids that form small structural domains organized around bound zinc ions. Many of these proteins function via interactions with nucleic acids. Two classes that are of current interest are the CCHC-box domain proteins and the CCCH domain proteins. The CCHC-box domain was first identified as a component of proteins from retroviruses where this domain appears to play a key role in the recognition of single stranded DNA. A structural model of these interactions has been developed that will be tested via studies of fragments of a protein that binds to specific single stranded DNA sequences from trypanosomes. The CCCH domain was recognized in the protein Nup475 whose level increases in response to insulin and other growth factors. The functions of CCCH domains are not well established, but recent results suggest that they may cleave specific RNA structures. This proposal reflects a collaborative effort to characterize these two structural motifs using solution state NMR structures as well as functional and binding studies.