PREF-1 FUNCTION IN ISLET GROWTH & DIFFERENTIATION

性状：（改编自申请人摘要）前脂肪细胞因子1 （EGFR- 1）是一种跨膜蛋白，含有表皮生长因子样蛋白， 重复。Pref- 1可以被切割以产生可溶性因子。使用 差异筛选，研究人员最初克隆pref-l从 胚胎来源的3 T3-L1细胞。相关的观察结果表明， 在胰岛β细胞的生长和分化过程中，具体 这项探索性的资助计划的目的是：1）展示生物多样性的功能 通过产生转基因小鼠， 过表达由胰岛素启动子驱动的β-l。2)为了检验 在AR 42 J细胞分化和生长过程中， 胰岛素阳性内分泌细胞。

DESCRIPTION: (Adapted from the applicant's abstract) Preadipocyte factor 1 (pref- 1) is a transmembrane protein that contains epidermal growth factor-like repeats. Pref- 1 can be cleaved to generate a soluble factor. Using differential screening ,the investigators originally cloned pref-l from embryonally- derived 3T3-Ll cells. The correlative observations suggest a role for pref- 1 during growth and differentiation of islet 3-cells. The specific aims of this exploratory grant proposal are: 1) To demonstrate pref- I function in 13-cell growth and differentiation by generating transgenic mice overexpressing pref- 1 driven by the insulin promoter. 2) To examine the effect of pref- 1 during in differentiation and growth of AR42J cells into insulin-positive endocrine cells.