INTEGRATION OF FTIR AND A CAPTIVE BUBBLE SURFACTOMETER

FTIR 与固定气泡表面计的集成

• 批准号: 6188516
• 负责人: JOHN E BAATZ
• 金额: $ 9.17万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-15 至 2002-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6188516
• 关键词: animal tissue; bioengineering /biomedical engineering; biomedical equipment development; biophysics; charge coupled device camera; digital imaging; infrared spectrometry; interferometry; intermolecular interaction; protein purification; protein structure function; pulmonary surfactants; surface property

Current methods, whether from instrumental sensitivity or design, have not been capable of effectively studying the structure and/or function of pulmonary surfactant proteins. It is crucial for the development of treatments for NRDS and ARDS that the mechanisms by which these proteins function be determined. This will be accomplished by combining a sensitive infrared spectroscopic technique (ATR-FTIR) with a unique captive bubble surfactometer (CBS) to yield a method that will be capable of defining lipid/protein interactions and surfactant protein structure of preparations that accurately mimic physiological conditions. The goals of the proposed research are to optimize parameters for FTIR spectral acquisition in the CBS/ATR-FTIR sample chamber, to optimize parameters and instrumentation for surface tension measurements by the CBS component, and to integrate all components of the CBS/ATR-FTIR sampling accessory for complete computerized instrumental control and data acquisition. This configuration will permit simultaneous measurement of 1) monolayer surface pressure, 2) protein structure (as a function of surface pressure), 3) protein and lipid orientation (vs. surface pressure), and 4) protein/lipid interactions of surfactant proteins and other proteins (e.g., membrane receptors, antimicrobial peptides) at a lipid monolayer interface. Simultaneous structure/function measurements by CBS/ATR-FTIR will make it possible to delineate the role of each surfactant protein in the biological function of pulmonary surfactant in healthy and diseased lungs. In the near future, experiments with more complex surfactant protein/lipid mixtures may aid in the improvement and development of treatments for surfactant deficient diseases in infants, children and adults.

当前的方法，无论是从工具灵敏度还是设计中，都无法有效研究肺表面活性剂蛋白的结构和/或功能。 对于开发NRD和ARD的处理至关重要，即确定这些蛋白质功能的机制。 这将通过将敏感的红外光谱技术（ATR-FTIR）与独特的圈养气泡表面仪（CBS）结合在一起来实现，从而产生一种能够定义脂质/蛋白质相互作用和表面活性剂蛋白质的制剂结构的方法，以准确地模拟生理条件。 拟议研究的目标是优化CBS/ATR-FTIR样品室中FTIR光谱采集的参数，以优化CBS组件的参数和仪器，以进行表面张力测量，并整合CBS/ATR-FTIR采样配件的CBS/ATR-FTR-FTER辅助配件的所有组件，以进行完整的计算机工具控制和数据采集。 这种配置将允许同时测量1）单层表面压力，2）蛋白质结构（作为表面压力的函数），3）蛋白质和脂质方向（与表面压力）和4）表面蛋白质和其他蛋白质的蛋白/脂质相互作用（例如 CBS/ATR-FTIR同时进行结构/功能测量将使您可以描述每种表面活性剂蛋白在健康和患病的肺中肺表面活性剂生物学功能中的作用。 在不久的将来，进行更复杂的表面活性剂蛋白/脂质混合物的实验可能有助于改善和发展婴儿，儿童和成人的表面活性剂缺乏疾病的治疗方法。