NEUROENDOCRINE PEPTIDES AND THEIR RECEPTORS REGULATING CORTICOTROPHES

促肾上腺皮质激素释放因子（CRF）是中枢神经系统的关键调节因子， 释放因子（CRF）是神经系统的关键神经调节因子， 介导许多内分泌、神经内分泌、自主神经和行为反应 介导许多内分泌、神经内分泌、自主神经和行为反应 肽及其结合位点，当考虑到许多生物 肽及其结合位点，当考虑到许多生物 中枢神经系统、免疫系统和其他系统。 CRF的行动是 在中枢神经免疫系统和其他系统中。通用报告格式的行动 在上一个资助期内， 第一个是在上一个资助期由这个项目克隆的 分布在垂体促皮质激素和整个中枢神经系统。 我们将 环化酶和分布在垂体促皮质激素和整个 受体和其他促皮质激素调节剂如糖皮质激素 受体和其他促皮质激素调节剂如糖皮质激素 配体介导的下调和脱敏机制将被 配体介导的下调和脱敏机制将 结合和信号产生以及调节受体敏感性 结合和信号产生以及调节受体敏感性 项目二，配体结构要求，结合到克隆 项目二结合克隆的配体结构要求 蛋白质，我们在过去的一段时间里也克隆和研究过。 一个 在过去的一段时间里，我们也克隆和研究了蛋白质。一个 我们将筛选编码该基因的cDNA， 我们将筛选编码该基因的cDNA， 将通过分子生物学技术鉴定或纯化 相关配体将通过分子生物学技术鉴定， 将被提纯并进行化学鉴定

Corticotropin releasing factor (CRF) is the key neuroregulatory of the releasing factor (CRF) is the key neuroregulator of the mediate many endocrine, neuroendocrine, autonomic and behavioral responses mediate many endocrine neuroendocrine autonomic and behavioral responses the peptide and its binding sites when considered with many biological the peptide and its binding sites when considered with many biological the central nervous, immune and other systems. The actions of CRF are within the central nervous immune and other systems. The actions of CRF of which was cloned by this project during the last grant period and shown first of which was cloned by this project during the last grant period distributed in pituitary corticotropes and throughout the CNS. We will cyclase and to be distributed in pituitary corticotropes and throughout receptor by CRF and other corticotrope regulators such as glucocorticoids receptor by CRF and other corticotrope regulators such as glucocorticoids mechanisms of ligand-mediated down regulation and desensitization will be mechanisms of ligand-mediated down regulation and desensitization will binding and signal generation and for regulation of receptor sensitivity binding and signal generation and for regulation of receptor sensitivity Project II, the ligand structural requirements for binding to the cloned Project II the ligand structural requirements for binding to the cloned protein, also cloned and studied by us during the past period. An protein also cloned and studied by us during the past period. An we will screen for cDNA's encoding this gene and will complete its analysis we will screen for cDNA s encoding this gene and will complete its will be identified by molecular biological techniques or will be purified related ligands will be identified by molecular biological techniques or will be purified and chemically characterized