PROTEIN/PEPTIDE AMINO ACID SEQUENCER

蛋白质/肽氨基酸测序仪

• 批准号: 2804059
• 负责人: CAROL J FIOL
• 金额: $ 11.59万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-06-01 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2804059
• 关键词: biomedical equipment; biomedical equipment purchase; computer assisted sequence analysis; protein sequence

This application is a joint effort of investigator sat Colorado State University (CSU) who engage in the study of protein structure and function, to obtain funding for the purchase of a state-of-the-art automated protein sequencer (PE Applied Biosystems Procise Model). The instrument will be housed and operated by Macromolecular Resource Facility (MRF) which is a shared instrumentation program at CSU that contains a protein core laboratory. Edman sequencing technology has been an essential tool for protein structure analysis at MRF. It is used synergistically with mass spectrometry methods to complete projects involving identification of proteins, de novo sequencing, characterization of post-translational modifications in proteins, and other protein/structure studies. The new sequenator will replace an old sequencer (PE-Applied Biosystems Model 473A) which after many years of productive use has become obsolete mainly due to the lack of available replace parts from the manufacturer. The new instrument will provide higher sensitivity, throughput and reliability. The major user group will take advantage of an established infrastructure that provides the necessary expertise and accessibility by placing the instrument in the MRF laboratory. Service will be offered with top priority, on a first come first served basis, to the major user group with a charge to cover the cost of instrument operation and maintenance. Other potential users within the academic community will be able to access the instrument on a secondary priority. The research interests of the investigators submitting this proposal cover a wide range of basic biological and health related problems including: infectious diseases, vaccine and drug development; transcriptional regulation and chromatin structure; neuronal cytoskeleton dynamics and signal transduction; and amino acid metabolism. The diversity of research interests of this user group and the extended research community at CSU will ensure that the benefits of this purchase will be far reaching in the areas of public health.

该申请是科罗拉多州立大学（CSU）研究人员的共同努力，他们从事蛋白质结构和功能的研究，以获得购买最先进的自动蛋白质测序仪（PE Applied Biosystems Procise Model）的资金。该仪器将由高分子资源设施（MRF）容纳和操作，MRF是CSU的共享仪器项目，包含蛋白质核心实验室。Edman测序技术已成为MRF蛋白质结构分析的重要工具。它与质谱方法协同使用，以完成涉及蛋白质鉴定、从头测序、蛋白质翻译后修饰表征和其他蛋白质/结构研究的项目。新的测序仪将取代旧的测序仪（PE-应用生物系统473 A型），该测序仪经过多年的生产使用后已经过时，主要原因是缺乏制造商提供的可用更换部件。新仪器将提供更高的灵敏度、通量和可靠性。主要用户群体将利用现有的基础设施，通过将仪器放置在MRF实验室，提供必要的专业知识和可访问性。服务将以先到先得的方式优先提供给主要用户群体，并收取仪器操作和维护费用。学术界的其他潜在用户将能够作为次要优先事项访问该文书。提交该提案的研究人员的研究兴趣涵盖了广泛的基本生物学和健康相关问题，包括：传染病，疫苗和药物开发;转录调控和染色质结构;神经元细胞骨架动力学和信号转导;和氨基酸代谢。该用户群的研究兴趣的多样性和CSU的扩展研究社区将确保此次购买的好处将在公共卫生领域产生深远影响。