AUTOMATED DNA SEQUENCER

该提案的目标是推进高速自动化DNA测序的基本设施服务，作为太平洋生物医学研究中心（PBRC）和少数民族机构计划研究中心（RCMI）的共同努力，以提高大学的健康相关和生物分子研究和研究生教育的能力。请拨资金购买一台配有XL升级翻译接口处理器的Perkin-Elmer ABI 377 DNA测序仪和相关软件。新的DNA测序仪将使生物技术/分子生物学仪器和培训设施（BMBITF）能够为许多正在进行的和新的研究项目提供最先进的仪器和技术，以保持最新的技术和开发新的支持服务。五年多前，BMBITF开始支持与健康相关的生物分子研究，用一台ABI 373 DNA测序仪来满足大学当时对测序的适度需求。从那时起，DNA测序已经取得了巨大的飞跃，成为这个校园许多研究项目的重要组成部分。对这项服务的需求正在以这样的速度增长，所涉及的工作量超出了现有核苷酸测序仪的能力。所要求的高通量测序仪器将消除研究界目前由于设施用户过载而经历的延迟。Perkin-Elmer公司的仪器和附件是目前满足该设施需要的最佳仪器，价格为150 000美元。在大学生物医学领域的研究小组在本地和快速获得序列数据的能力是保持其竞争地位，获得校外资金的重要。该提案重点介绍了NIH支持的9个具有代表性的研究项目，以详细展示该仪器的未来用途。这些建议如下：人类蜕膜和胎儿膜作为一个Paracryne系统，2。弹性假黄瘤中的弹性蛋白基因; 3.基质：产前和产后弹性组织的调节; 4.结肠癌赖氨酰氧化酶基因突变的研究; 5.弹性基因突变与血管上主动脉狭窄; 6.共生的费氏弧菌在上皮细胞中的定殖; 7. RSV感染细胞中连接蛋白45磷酸化; 8.大鼠人松弛素受体基因的克隆; 9.扩张对人胎膜的影响。所要求的工具还将用于合格学生的学术培训，主要是参加PBRC RCMI方案的学生。

The goal of this proposal is to advance essential facility services in high speed automated DNA sequencing as a joint effort of the Pacific Biomedical Research Center (PBRC) and the Research Centers in Minority Institutions Program (RCMI) to increase the University's capability for health-related and biomolecular research and graduate education. Funds are requested to purchase a Perkin-Elmer ABI 377 DNA Sequencer with XL Upgrade Translation Interface Processor and associated software. The new DNA sequencer will enable the Biotechnology/Molecular Biology Instrumentation and Training Facility (BMBITF) to provide state-of-the- art instrumentation and techniques for many ongoing and new research projects, to stay current on technology and develop new supporting services. Over five years ago, the BMBITF begin supporting health- related and biomolecular research with a single ABI 373 DNA sequencer to meet the University's then modest demand for sequencing. Since then, DNA sequencing has taken a quantum leap and become an essential element in many research programs on this campus. The demand for this service is increasing at such a rate that the volume of work involved outruns the capacity of the existing nucleotide sequencer. The requested high- throughput sequencing instrument would eliminate the delays that the research community presently experiences due to facility user-overload. The instrumentation and accessories from Perkin-Elmer, which at the present time is the best instrument for the needs of the Facility, costs $150,000. The ability of research groups in biomedical areas at the University to obtain sequence data locally and rapidly is important for maintaining their competitive position for obtaining extramural funding. This proposal focuses on nine representative NIH-supported research projects in detail to demonstrate the future use of the instrument. These are as follows: I. Human Decidua and Fetal Membranes as a Paracryne system, 2. Elastin Gene in Pseudoxanthoma Elasticum; 3. Matrix: Regulation in Pre- and Postnatal Elastic Tissues; 4. Lysyl Oxydase Gene Mutations in Colon Cancer; 5. Elastic Gene Mutations in Supravascular Aortic Stenosis; 6. Colonization of Epithelium by Symbiotic Vibrio Fischeri; 7. Connexin 45 Phosphorylation in RSV- Infected Cells; 8. Cloning of the Rat Human Relax Receptor Genes; 9. The Effects of Distension on the Human Fetal Membranes. The requested instrument will also be used in academic training of qualified students, primarily those participating in the RCMI Program of PBRC.