MULTIUSER EPR SPECTROMETER AT UCSD

UCSD 多用户 EPR 光谱仪

• 批准号: 2766819
• 负责人: MARK H THIEMENS
• 金额: $ 39.06万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-16 至 2000-02-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2766819
• 关键词: biomedical equipment; biomedical equipment purchase; electron spin resonance spectroscopy

This is a revised request from the University of California at San Diego for funds to purchase an advanced electron paramagnetic resonance (EPR) spectrometer. A User's Group has been established at UCSD involving 8 faculty and staff from 4 UCSD campus departments and 1 staff at The Scripps Research Institute; 8 NIH funded PI's are represented. The individual research activities to be supported by the purchase of the spectrometer include studies of metal binding sites in heme proteins and proteins of respiration and photosynthesis, models for metalloprotein clusters and metal quinone complexes, conformational changes in enzymes subject to complex regulatory signals, assembly of molecular aggregates on or within lipid bilayers, interactions of lipids with membrane proteins and the photochemical conversion of Green Fluroescent Protein to a form that emits red light. The spectrometer of choice is the Bruker ELEXSYS system, operating at X-band frequency. Justifications for the choice of this instrument include its state-of-the-art capabilities as a continuous wave spectrometer and its capacity to be expanded (with future additional funding) to carry out more advanced EPR experiments, such as multifrequency EPR, ENDOR and ESEEM. We anticipate a future need for such experiments. Funds are also requested for the purchase of appropriate cryogenic apparatus, a TM110 mode cavity for more sensitive recording of protein bound spin-labels and an NMR gaussmeter to obtain accurate g-values. The total cost of the system is 390,571 dollars. An Administrative Arrangement and a Long-Term Operation Plan are presented.

这是圣地亚哥加州大学为购买先进的电子顺磁共振（EPR）光谱仪而提出的修订请求。 一个用户小组已经在加州大学圣地亚哥分校成立，包括来自加州大学圣地亚哥分校4个校园部门的8名教职员工和斯克里普斯研究所的1名工作人员; 8名NIH资助的PI代表。 购买光谱仪支持的个人研究活动包括血红素蛋白和呼吸和光合作用蛋白中的金属结合位点的研究，金属蛋白簇和金属醌复合物的模型，受复杂调控信号影响的酶的构象变化，脂质双层上或脂质双层内分子聚集体的组装，脂质与膜蛋白的相互作用以及绿色荧光蛋白向发射红光的形式的光化学转化。 所选的光谱仪是Bruker ELEXERAND系统，在X波段频率下工作。 选择该仪器的理由包括其作为连续波光谱仪的最先进的能力，以及其进行更先进的EPR实验（如多频EPR，ENDOR和ESEEM）的能力（未来有额外的资金）。 我们预计将来需要进行这种实验。 还要求提供资金购买适当的低温设备、一个TM 110模腔以更灵敏地记录蛋白质结合的自旋标记和一个核磁共振高斯计以获得准确的g值。 该系统的总成本为390，571美元。 提出了行政安排和长期运营计划。