CORE--MOLECULAR ANALYSIS

核心--分子分析

• 批准号: 6100611
• 负责人: ROBERT A SWERLICK
• 金额: $ 6.87万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6100611
• 关键词: analytical chemistry; biomedical facility; chemical synthesis; electrospray ionization mass spectrometry; flow cytometry; immunofluorescence technique; mass spectrometry; peptide chemical synthesis; protein sequence; reagent /indicator; skin

Basic science oriented skin centered research that is both comprehensive and mechanistic requires the use of multiple investigative tools and techniques. It is extremely difficult for any single laboratory to develop all the necessary tools to fully address complicated issues concerning skin biology and skin related disease. Equipment required is likely to be prohibitively expensive for any single investigator to acquire. Additionally, the optimal operation of sophisticated equipment or execution of difficult assays is best done by dedicated experienced personnel. The Molecular Analysis Core will provide a wide variety of services, expertise, and reagents to ESDRC investigators which will facilitate their studies. The Cell Adhesion Component will provide specialize matrix proteins, will help design and execute cell adhesion experiments using both static and flow systems, and will provide for investigative methodologies which explore the effects of fluid flow dynamics upon cell membrane signalling and physiologic and pathophysiologic responses. The Microchemical Component will provide specialized peptides and oligonucleotides, protein sequencing, and HPLC. The Flow cytometric/Cell Sorting Component will provide multiparameter flow cytometric analysis, cell sorting, and cell cycle analysis. This core will provide ESDRC investigators with an invaluable resources which will clearly facilitate investigations into basic skin biology and foster collaborative investigations across multidisciplinary fields of research.

以基础科学为导向，以皮肤为中心的研究，既是综合性的 机械性要求使用多种调查工具和 技巧。对于任何一个实验室来说，要想让 开发所有必要的工具以全面解决复杂问题 关于皮肤生物学和皮肤相关疾病。所需设备为 对于任何一名调查员来说， 收购。此外，尖端设备的优化运行 或者，执行困难的分析最好是由经验丰富的专业人员完成 人事部。分子分析核心将提供广泛的 向ESDRC调查人员提供服务、专业知识和试剂 为他们的学习提供便利。细胞黏附组件将提供 专门化基质蛋白，将帮助设计和执行细胞黏附 使用静态和流动系统的实验，并将提供 探索流体流动影响的调查方法 细胞膜信号转导与生理和免疫功能的动力学 病理生理反应。微量化学成分将提供 专化肽和寡核苷酸、蛋白质测序和高效液相色谱分析。 流式细胞仪/细胞分选组件将提供多参数 流式细胞仪分析、细胞分类和细胞周期分析。这 CORE将为ESDRC调查人员提供宝贵的资源 将明显促进对基本皮肤生物学的研究并促进 跨多学科研究领域的协作调查。