PROPIONATE UTILIZATION, GLUCONEOGENESIS, & PYRUVATE RECYCLING IN HUMAN LIVER

丙酸利用、糖异生、

• 批准号: 6205892
• 负责人: ROLF GRUETTER
• 金额: $ 0.7万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-15 至 2000-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6205892
• 关键词: Mammalia; bioimaging /biomedical imaging; biomedical resource; human tissue; liver; magnetic resonance imaging; minerals; urinary tract

Dr. Bansal has recently moved to the University of Pennsylvania. Upon completion of several manuscripts which are in preparation, this subpoject will be deleted. We have implemented a three-dimensional 23Na MQ imaging sequence on the 4.7T system. This gradient echo imaging sequence is capable of collecting interleaved SQ and TQ 23Na images with very short echo times (2.5-5 ms). We have used this imaging sequence to study the effect of ischemia in rabbit kidney. Interleaved SQ and TQ filtered 23Na images were collected before and after sacrificing the animal with 32 x 32 x 64 data points over a 50 x 50 x 100 mm3 field of view and 4 ms echo time. The total image data collection time was 4 hours. The SQ image indicates a 4-5 fold greater signal intensity in the medullary region as compared to the cortex. The TQ filtered image of the normal kidney did not show any signal intensity due to the low sensitivity of the technique. However, on death, the renal cortex became clearly visible in the TQ filtered image without any substantial increase in signal intensity in the medullary region. This is mainly because the cortical region of the kidney has a large intracellular space while the medullary region is mainly intraluminal and vascular. These images clearly suggest that increase in MQ filtered signal intensity may be a very good marker of cellular injury. (Service 3) REPORT PERIOD: (09/01/97-08/31/98)

Bansal博士最近搬到了宾夕法尼亚大学。 在完成几个正在编写的手稿后， 主题将被删除。 我们实施了一个三维的 4.7T系统上的23 Na MQ成像序列。 这个梯度回波 成像序列能够收集交错的SQ和TQ 23 Na 回波时间非常短（2.5-5 ms）的图像。 我们已经用这种 成像序列，以研究兔肾缺血的影响。 之前收集交错SQ和TQ过滤的23 Na图像， 在处死动物后，在50 × 32 × 64个数据点上， 50 x 100 mm 3视野和4 ms回波时间。 总图像数据 收集时间为4小时。 SQ图像显示4-5倍 在髓区的信号强度更大， 皮层 正常肾脏的TQ滤波图像未显示任何 由于该技术的低灵敏度，信号强度降低。 然而，在死亡时，肾皮质在TQ中变得清晰可见 滤波图像中没有任何实质性的信号强度增加， 髓区 这主要是因为大脑皮层 肾脏具有大的细胞内空间，而髓质区域 主要是管腔内和血管。 这些图像清楚地表明 MQ滤波信号强度增加可能是非常好的 细胞损伤的标志物。 （服务3）报告期： (09（1997年1月至1998年8月31日）