TYROSINE FLUORESCENCE STUDIES W/ DERMORPHIN & L ALA DERMORPHIN

流式细胞术荧光偏振q FITC，BSA（x = 5，10， 或20）和FITC-聚-D-酪氨酸激酶探针在混合微噬菌体中显示了 与时间和抗原浓度相关的一定趋势 BSA探针的表位密度。 为了研究这种趋势是否 由于体外细胞内抗原的还原和解折叠 测量相同探针的极化。 还原和展开 的FITC-BSA显示出由于开放的极化降低， BSA分子和更大的运动。 观察到类似的效果， FITC-聚-D-赖氨酸。

Flow cytometric fluorescence polarization q FITC, BSA (x = 5, 10, or 20) and FITC-poly-D-tykine probes in mixing microphage showed a certain trend with time and concentration of antigen that correlated to epitope density of the BSA probes. To study whether this trend was due to reduction and unfolding of the antigen inside the cell in-vitro polarigation of the same probes was measured. Reduction and unfolding of FITC-BSA showed a decrease in polarization due to opening up of the BSA molecule and greater motion. A similar effect was observed with FITC-poly-D-lysine.