WASTE SITE ASSESSMENT

废物场评估

• 批准号: 6106230
• 负责人: KIRK Warren BROWN
• 金额: $ 11.82万
• 依托单位: TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-05 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6106230
• 关键词: air pollution; air sampling /monitoring; bioassay; biotransformation; carbopolycyclic compound; environmental contamination; environmental toxicology; ground water; halohydrocarbon; industrial waste; mutagen testing; mutagens; soil pollution; soil sampling; toxicant interaction; toxicant screening; waste disposal; water pollution; water sampling /testing

Description: The objective of the renewal project is to develop a comprehensive procedure to assess the human health risk associated with a Superfund site. Biological testing will provide data to define the immunotoxic, genotoxic, developmental and neurotoxic potential of environmental samples. Five experiments will be conducted as part of this project to evaluate the utility of a biologically-based protocol to estimate human health risk at a Superfund site. The first two experiments will assess the risk associated with complex mixtures from Superfund sites as well as model compounds and reconstituted mixtures representative of the AH and HAH chemicals. Experiment 1 is designed to compare estimates of human health risk using either biological or chemical analysis. This experiment will involve the collection of samples from Superfund sites and matched control areas. Environmental samples will be collected from a broad range of Superfund sites representative of AH (i.e. refinery waste, coal gasification waste) and HAH (i.e. wood-preserving waste, PCB contaminated soils and sediments) contaminants. An initial screen of site samples will be performed using an acute toxicity and two microbial genotoxicity assays. Selected samples will also be subjected to a quantitative GC/MS analysis. Based on the results of the preliminary screen, a limited number of samples will be analyzed in the battery of toxicological tests developed in Projects 1 through 6. A second experiment will be conducted to fractionate and isolate the most toxic components from selected complex mixtures. Isolated components will be reconstituted for testing (as individual compounds and mixtures) in the bioassays to evaluate the potential interactions of the components of a complex mixture. The data from these experiments will be used to provide biological test data describing the interactions of the components of complex mixtures and their potential toxic equivalency factors (TEFs). The third and fourth experiments will focus on monitoring detoxification of complex mixtures. These experiments will provide information to define the ability of remedial techniques to reduce toxicity and the potential threat to human health of complex mixtures in the environment. In the fifth experiment, phyllosilicate clays (natural and chemically-modified) will be evaluated for their abilities to tightly bind and detoxify target chemicals. The sites and molecular mechanisms of chemisorption will be elucidated.

描述：更新项目的目的是开发 评估与人类健康风险相关的全面程序 超级基金网站。生物测试将提供数据以定义 免疫毒性，遗传毒性，发育和神经毒性潜力 环境样品。将进行五个实验作为一部分 该项目旨在评估基于生物学方案的实用性 估计超级基金站点的人类健康风险。前两个 实验将评估与复杂混合物相关的风险 超级基金站点以及模型化合物和重构混合物 AH和HAH化学物质的代表。实验1是设计的 比较使用生物学或 化学分析。该实验将涉及 来自超级基金站点和匹配的控制区域的样品。环境的 样品将从各种超级基金站点收集 AH的代表（即炼油厂废物，煤气废物）和 哈（即保护木材的废物，PCB污染的土壤和沉积物） 污染物。使用站点样本的初始屏幕将使用 急性毒性和两个微生物遗传毒性测定。选定 样品还将进行定量GC/MS分析。基于 在初步屏幕的结果中，样本数量有限 将在开发的毒理学测试电池中进行分析 项目1至6。将进行第二次实验 分馏和分离从选定复合物中的最毒性成分 混合物。孤立的组件将被重组进行测试（AS 在生物测定中的单个化合物和混合物）以评估 复杂混合物的组件的潜在相互作用。数据 这些实验将用于提供生物测试数据 描述复杂混合物和 它们的潜在有毒等效因素（TEF）。第三和第四 实验将集中于监测复杂混合物的排毒。 这些实验将提供信息以定义 降低毒性和对人的潜在威胁的补救技术 环境中复杂混合物的健康。在第五实验中， 将评估植物硅酸盐粘土（天然和化学修饰） 因为它们能够紧密结合和排毒目标化学物质。这 将阐明化学吸附的位点和分子机制。