CORE--FITCH MONOCLONAL ANTIBODY FACILITY

惠誉单抗设备是一个共享资源，有两个 主要目标：(1)为芝加哥大学提供研究人员 癌症研究中心和芝加哥大学的其他研究人员 高度可及、经济实惠的高质量发展方式 临床或临床用的单抗和多克隆抗体 使用经过验证的方法进行基础研究；(2)此外，我们还 与调查人员密切合作，开发新的方法来产生 优质的单克隆抗体将成为诊断的有用试剂 和心理治疗。该设施目前为调查人员提供指导 以及在开发试剂方面的技术专长 他们的具体研究需求。我们还提供旨在 提高在这里或其他地方开发的试剂的有用性，包括 杂交瘤亚克隆、单抗同工分型或定量， 腹水产生或其他产生适度水平的 用于研究的抗体建议。我们正在与The Animal合作 资源中心在开发高效体外培养方法中的应用 抗体的产生。 单抗已经成为治疗艾滋病的重要工具。 生物学研究的跨学科方法，以及 需要单抗的应用领域继续扩大，因为 这项技术已经发展起来了。单抗仍然非常强大 癌症研究中细胞免疫表型的工具，移植， 和免疫学。它在生物化学中的用途已经扩大了 分析这些细胞和其他细胞的正常和异常功能。 此外，在许多情况下，单抗都是关键因素。 诊断和治疗的实验和临床策略 某些疾病。我们衍生和生产单抗的能力 提供高度可访问的资源，以满足日益增长的 这样的试剂在癌症中心的研究社区。

The Fitch Monoclonal Antibody Facility is a shared resource with two major goals: (1) to provide investigators in the University of Chicago Cancer Research Center and other University of Chicago investigators with a highly accessible and economical means for developing high-quality monoclonal and polyclonal antibodies for the purposes of clinical or basic research using proven methodologies; (2) in addition we also work closely with the investigators to develop novel approaches for generating quality monoclonal antibodies that will be useful reagents in diagnosis and therapy. The facility presently provides investigators with guidance and technical expertise in developing reagents that are well suited to their specific research needs. We also provide services designed to augment the usefulness of reagents developed here or elsewhere, including hybridoma subcloning, monoclonal antibody isotyping or quantitation, ascites production or other methods of generating moderate levels of antibody for research proposes. We are collaborating with the Animal Resources Center in the development of efficient in vitro methods of antibody production. Monoclonal antibodies have become essential tools in the interdisciplinary approaches to biological research, and the variety of applications requiring monoclonal antibodies have continued to expand as this technology has developed. Monoclonal antibodies remain very powerful tool for immunophenotyping cells in the study of cancer, transplantation, and immunology. There usefulness has been extended in the biochemical analysis of normal and abnormal functions of these and other cells. Furthermore, monoclonal antibodies are pivotal elements in many experimental and clinical strategies for the diagnosis and treatment of certain diseases. Our ability to derive and produce monoclonal antibodies provides a highly accessible resource which meets a growing demand for such reagents in the Cancer Center research community.