STRUCTURE & FUNCTION OF HELICASES: DNA REPLICATION

结构

• 批准号: 6120559
• 负责人: MONTSERRAT BARCENA
• 金额: $ 0.3万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120559
• 关键词: Chordata; Invertebrata; biomedical resource; genetics; growth /development; reproductive system

Ile structure of a cloned Drosophila nuclear protein which can catalyze the decondensation of demembranated Xenopus sperm chromatin (chromatin remodeling protein, CRP 1) was studied in the STEM to compare it to Xenopus nucleoplasmin to which it is quite homologous in amino acid sequence. While most evidence suggests that Xenopus nucleoplasmin is pentameric, mass analysis from the STEM suggests that CRP I is tetrameric (while Xenopus nucleoplasmin is pentameric), in agreement with chemical crosslinking experiments. However, negatively stained CRP I and Xenopus nucleoplasmin were morphologically indistinguishable in the STEM. (Crevel et al., 1996). The structure of CRP2, a second heat-stable chromatin. remodeling protein of Drosophild which appears to be able to substitute in a mutant knocking out CRP 1, is currently under investigation. Very large aggregates have been observed.

克隆果蝇核蛋白的 Ile 结构 催化非洲爪蟾精子染色质脱膜解缩 （染色质重塑蛋白，CRP 1）在 STEM 中进行了研究 将其与爪蟾核蓝蛋白进行比较，其在以下方面非常同源： 氨基酸序列。 虽然大多数证据表明非洲爪蟾 核蓝蛋白是五聚体，STEM 的质量分析表明 CRP I 是四聚体（而非洲爪蟾核浆蛋白是五聚体）， 与化学交联实验一致。 然而，消极地 染色的 CRP I 和非洲爪蟾核蓝蛋白在形态学上 在STEM中无法区分。 （Crevel 等人，1996）。 结构 CRP2，第二个热稳定染色质。 重塑蛋白 果蝇似乎能够替代突变体敲除 CRP 1，目前正在调查中。 非常大的聚集体 已被观察到。