METABOLIC MEASUREMENT IN LYMPHOMA TREATED WITH CHEMOTHERAPY

我们将使用[C-11]标记的胸腺嘧啶核苷，以及其他测量肿瘤的方法 新陈代谢(血流量，血容量)，以检验假设在 淋巴瘤代谢的活体测量将与对 化疗。这些测量将在测量前和测量期间获得 对狗和人类都有治疗作用。我们将检验这样的假设：我们的 测量有效的基础上的替代测量在消费税 肿瘤，以及通过重复测量。例如，[C-11]- PET测量的胸腺嘧啶核苷摄取将与直接 在提取的组织中掺入DNA。这其中的一个重要目标 工作是确定各种新陈代谢之间的关系 测量。初步研究将集中在测量胸腺嘧啶核苷。 新陈代谢、血流量和血量，因为这些都是需要的 准确模拟胸腺嘧啶核苷在组织中的传递和滞留。 随着它们的发展，对能量学的测量由 葡萄糖代谢将与胸腺嘧啶核苷的测量结果进行比较。 新陈代谢。肿瘤血流量和血容量的测量也将 应用于淋巴瘤患者接受治疗评估的研究 用放射性标记的单抗。我们发现了很大的变异性 在这类患者对放射性标记抗体的摄取方面，这 变异性不能用抗原密度的差异来解释 肿瘤。因此，我们将检验变量肿瘤血液的假设 用[O-15]-水和[Ga-]-水测量的流量和渗透率 68、转铁蛋白分别占抗体差异的原因 领悟。

We will use [C-11]-labeled thymidine, along with other measures of tumor metabolism (blood flow, blood volume), to test the hypothesis that in vivo measurements of lymphoma metabolism will correlate with response to chemotherapy. These measurements will be obtained before and during therapy in both dogs and humans. We will test the hypothesis that our measurements are valid based on alternative measurements in excised tumors, as well as by replicate measurements. For example, [C-11]- thymidine uptake measured by PET will be compared with direct incorporation into DNA in extracted tissue. One important goal of this work is to determine the relationship between the various metabolic measurements. Initial studies will concentrate on measuring thymidine metabolism, blood flow, and blood volume, since these will be needed to accurately model the delivery and retention of thymidine in the tissue. As they are developed, measurements of energetics as determined by glucose metabolism will be compared to the measurements of thymidine metabolism. Measurements of tumor blood flow and blood volume will also be applied to the study of lymphoma patients being evaluated for therapy with radiolabeled monoclonal antibodies. We have found wide variability in the uptake of radiolabeled antibodies in such patients, and this variability is not explained by differences in antigen density on the tumors. Therefore, we will test the hypothesis that variable tumor blood flow and permeability, as measured with [O-15]-water and[Ga- 68]transferrin respectively, account for the differences in antibody uptake.