METABOLIC MEASUREMENT IN LYMPHOMA TREATED WITH CHEMOTHERAPY

我们将使用[C-11]标记的胸苷，沿着其他肿瘤指标 代谢（血流量，血容量），以检验假设， 淋巴瘤代谢的体内测量将与对 化疗这些测量结果将在 治疗在狗和人类。我们将测试我们的假设， 基于切除的替代测量值， 肿瘤，以及通过重复测量。例如，[C-11]- 通过PET测量的胸苷摄取将与直接 在提取的组织中掺入DNA。其中一个重要的目标是 工作是确定各种代谢之间的关系， 测量.最初的研究将集中在测量胸苷 代谢、血流量和血容量，因为这些将需要 准确地模拟胸苷在组织中的递送和保留。 随着它们的发展，能量学的测量， 将葡萄糖代谢与胸苷的测量进行比较 新陈代谢.肿瘤血流量和血容量的测量也将 应用于淋巴瘤患者的治疗评估研究 放射性标记的单克隆抗体。我们发现了很大的变异性 在这些患者中放射性标记抗体的摄取中， 变异性不能用抗原密度的差异来解释。 肿瘤的因此，我们将检验可变肿瘤血液 流动性和渗透性，如用[O-15]-水和[Ga- 68]转铁蛋白分别占抗体的差异 摄取。

We will use [C-11]-labeled thymidine, along with other measures of tumor metabolism (blood flow, blood volume), to test the hypothesis that in vivo measurements of lymphoma metabolism will correlate with response to chemotherapy. These measurements will be obtained before and during therapy in both dogs and humans. We will test the hypothesis that our measurements are valid based on alternative measurements in excised tumors, as well as by replicate measurements. For example, [C-11]- thymidine uptake measured by PET will be compared with direct incorporation into DNA in extracted tissue. One important goal of this work is to determine the relationship between the various metabolic measurements. Initial studies will concentrate on measuring thymidine metabolism, blood flow, and blood volume, since these will be needed to accurately model the delivery and retention of thymidine in the tissue. As they are developed, measurements of energetics as determined by glucose metabolism will be compared to the measurements of thymidine metabolism. Measurements of tumor blood flow and blood volume will also be applied to the study of lymphoma patients being evaluated for therapy with radiolabeled monoclonal antibodies. We have found wide variability in the uptake of radiolabeled antibodies in such patients, and this variability is not explained by differences in antigen density on the tumors. Therefore, we will test the hypothesis that variable tumor blood flow and permeability, as measured with [O-15]-water and[Ga- 68]transferrin respectively, account for the differences in antibody uptake.