ALTERED MECHANISMS OF WOUND REPAIR IN THERMAL BURNS

热烧伤伤口修复机制的改变

• 批准号: 6240392
• 负责人: FREDERICK GRINNELL
• 金额: $ 16.43万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至 1998-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6240392
• 关键词: SDS polyacrylamide gel electrophoresis; burn therapy; burns; elastase inhibitor; elastases; endopeptidases; exudate /transudate; fibronectins; growth factor; human subject; metalloendopeptidases; protease inhibitor; skin ulcer; surgical wound; western blottings; wound healing

The goal of this research program is to understand wound healing and its failure. In preliminary experiments, we analyzed wound fluid from burn wounds, chronic skin ulcers, and mastectomy wounds. The results showed that wound fluid can be used as a non-invasive method to learn about wound repair. During the next four years, we will build on the preliminary studies to answer important questions about the proteinases, adhesion proteins, and growth factors found in would fluid and their biological functions. Specific aims of the project are as follows: to learn if elastase is responsible for degradation of fibronectin in burn wound fluid and chronic ulcer fluid and if there is a defect in the proteinase inhibitor system; to learn if fibronectin fragments in wound fluid stimulate proteinase secretion or have endogenous proteinase activity; to learn if "activated" metalloproteinases in chronic ulcer and burn wound fluid are biologically active; to learn if treatment of mastectomy fluid with "inflammatory" enzymes results in metalloproteinase activation and fibronectin degradation similar to that seen in chronic wound fluid or burn wound fluid; to identify the burn wound fluid growth factor and other factors that modify keratinocyte migration or fibroblast contraction; and to further correlate the relationship between wound fluid composition and clinical healing.

这项研究计划的目标是了解伤口愈合及其 失败 在初步实验中，我们分析了烧伤的伤口液， 伤口、慢性皮肤溃疡和乳房切除术伤口。 结果表明 伤口液可以作为一种非侵入性的方法来了解伤口 修复. 在今后四年中，我们将在初步 研究来回答关于蛋白酶、粘附 蛋白质和生长因子，以及它们的生物活性。 功能协调发展的 该项目的具体目标如下： 弹性蛋白酶负责烧伤创面液中纤维连接蛋白的降解 和慢性溃疡液，如果蛋白酶有缺陷， 抑制剂系统;了解伤口液中是否存在纤维连接蛋白碎片 刺激蛋白酶分泌或具有内源性蛋白酶活性; 探讨慢性溃疡和烧伤创面是否存在“活化”金属蛋白酶 液体具有生物活性;了解乳房切除术液体的治疗 与“炎性”酶一起导致金属蛋白酶活化， 纤连蛋白降解类似于慢性伤口液中所见， 烧伤创面液;确定烧伤创面液生长因子和其他 改变角质形成细胞迁移或成纤维细胞收缩的因子;和 为了进一步关联伤口流体成分和 临床治愈