CORE--TISSUE CULTURE LABORATORY

核心--组织培养实验室

• 批准号: 6235896
• 负责人: JOANN M MOULDS
• 金额: $ 18.67万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-15 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6235896
• 关键词: biomedical facility; cell line; cell transformation; human genetic material tag; scleroderma; tissue /cell culture

A Tissue Culture Core will prepare, store and process for genomic DNA, Epstein-Barr virus (EBV) transformed lymphoblastoid cell lines from the Choctaw cohort (Project 1) and from SSc patients from three other ethnic groups (whites, African-Americans, and Mexican-Americans) for the genetic studies proposed in projects 1 and 4. Human skin fibroblasts from SSc patients for use in Project 1 and possibly Project 4, and human pulmonary macrophages for use in Project 3, also will be processed and stored in this central core. The EBV-transformed cell lines also will serve as a national resource for future studies of well-characterized patients with SSc (and multiplex families when identified). Dr. Joann M. Moulds will direct this core and be assisted by a Research Assistant II.

组织培养核心将准备，储存和处理基因组DNA， Epstein-Barr病毒（EBV）转化的淋巴母细胞系来自 乔托队列（项目1）和来自其他三个种族的SSc患者 群体（白人，非洲裔美国人和墨西哥裔美国人） 项目1和项目4中提出的遗传学研究。人皮肤成纤维细胞 用于项目1和可能的项目4的SSc患者，以及人类 用于项目3的肺巨噬细胞也将被处理， 储存在这个中央核心EBV转化的细胞系也将 作为未来研究的国家资源， SSc患者（以及确定的多重家族）。 博士Joann M.模具将指导这一核心，并由一个研究 二级助理。