CORE--MORPHOLOGY AND MORPHOMETRY

核心——形态学和形态学

• 批准号: 6242313
• 负责人: MARGARET W LEIGH
• 金额: $ 13.87万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6242313
• 关键词: apoptosis; biomedical facility; cytology; histochemistry /cytochemistry; histology; image processing; immunocytochemistry; in situ hybridization; light microscopy; morphology; proliferating cell nuclear antigen; transmission electron microscopy

The Morphology/Morphometry Core laboratory is designed to facilitate performance of morphologic, immunohistochemical, in situ hybridization, and morphometric studies needed for the proposed projects. This Core will provide service, consultation, technical assistance, and or training morphologic and imaging techniques for PPG investigators conducting cell biological and morphologic studies at the histologic and ultrastructural level. This laboratory maintains a full range of light and electron microscopes, ancillary apparatus and preparative resources as well as hardware and software for commuter-assisted image, morphometric, and densitometric analyses. The laboratory is staffed by well-qualified, experienced technicians who will process tissues for light and transmission electron microscopy including immuno- and other histochemical staining and in situ hybridization analyses. The Core director will perform the majority of the morphometric analyses needed by projects II and III. Two key consultants for this Core include Dallas Hyde, Ph.D. who provides expertise in stereology and morphometry of the lung and David Millhorn, Ph.D. who provides expertise in in situ hybridization. All of the major projects and most of the Pilot and Feasibility projects will rely heavily on this unit. This Core's diversity and cost-effectiveness are directly attributable to shared support with other research units using this facility, including a Pediatric Pulmonary SCOR, CF SCOR, and the Center for Environmental Medicine and Lung Biology.

形态学/形态测量学核心实验室旨在促进 形态学、免疫组化、原位杂交、 以及拟议项目所需的形态测量研究。这一核心将 提供服务、咨询、技术援助和/或培训 形态学和成像技术，用于PPG研究人员进行细胞 组织学和超微结构的生物学和形态学研究 水平这个实验室维持着全方位的光和电子 显微镜、辅助设备和制备资源，以及 用于通勤辅助成像、形态测量和 光密度分析该实验室的工作人员素质良好， 经验丰富的技术人员将处理组织的光， 透射电子显微镜检查，包括免疫和其他组织化学 染色和原位杂交分析。核心主管将 执行项目II所需的大部分形态测定分析 和三.该核心的两名关键顾问包括达拉斯海德博士。谁 提供了肺的体视学和形态测量学方面的专业知识，大卫 米尔霍恩博士他提供原位杂交的专业知识。所有 主要项目以及大多数试点和可行性项目将 非常依赖这个单位。该核心的多样性和成本效益 直接归因于与其他研究单位的共同支持 使用该设施，包括儿科肺SCOR，CF SCOR，以及 环境医学和肺生物学中心