OVARIAN STEROID REGULATION OF ESTROGEN & PROGESTIN RECEPTOR MRNA IN HYPOTHALAMUS

雌激素（ER）和孕激素受体（PR）mRNA的调节 在下丘脑中检查了它们的同源激素和蛋白质 和脑垂体的变化。 恒河猴（Macaca mulatta）被切除卵巢和卵巢切除（切除卵巢）， 植入含雌二醇17（E）的硅橡胶胶囊，或 孕酮（P）。 雌激素对乳腺癌雌激素受体和孕激素受体mRNA水平的调节 用原位杂交技术检测下丘脑和垂体 （ISH）。 在下丘脑中，ER和PR免疫检测蛋白是 在附近的部分也进行了检查。 在垂体中，mRNA水平是 与之前相同处理的ER和PR蛋白分析相比， 动物 E处理诱导下丘脑内侧基底核PR mRNA表达 和垂体。 补磷处理对PR mRNA表达无影响 水平，但明显减少PR mRNA在下丘脑， 脑垂体 与PR蛋白检测结果有很好的一致性， 免疫细胞化学（ICC）。 E处理对ER mRNA表达无影响。 下丘脑或脑垂体。 补充磷处理降低ER mRNA在腹内侧核（VMN）中表达，而不在弓状核中表达 或脑垂体中。 ER mRNA表达与ER 蛋白质在这些领域 总之，存在细胞特异性调节 下丘脑和垂体中P下调PR， 垂体PR而不影响ER。 P没有 显著影响PR在下丘脑的表达，即使P 降低VMN中的ER。 尽管这些观察表明， 细胞特异性调节机制，它们与ER一致， PR介导的生理事件，如催乳素分泌和 性行为

The regulation of estrogen (ER) and progestin receptor (PR) mRNA and protein by their cognate hormones was examined in the hypothalamus and pituitary of steroid treated monkeys. Rhesus macaques (Macaca mulatta) were ovariectomized and hysterectomized (spayed) and implanted with Silastic capsules containing estradiol 17 (E) or progesterone (P). Steroid regulation of ER and PR mRNA levels in the hypothalamus and pituitary was examined with in situ hybridization (ISH). In the hypothalamus, ER and PR immunodetectable proteins were also examined in nearby sections. In the pituitary, mRNA levels were compared to previous ER and PR protein analysis of identically treated animals. E treatment induced PR mRNA in the medial basal hypothalamus and pituitary. Supplemental P treatment had no effect on PR mRNA levels in the hypothalamus, but markedly reduced PR mRNA in the pituitary. There was excellent agreement with PR protein detection by immunocytochemistry (ICC). E treatment had no effect on ER mRNA in the hypothalamus or pituitary. Supplemental P treatment decreased ER mRNA in the ventromedial nucleus (VMN), but not in the arcuate nucleus or in the pituitary. There was agreement between ER mRNA and ER protein in these areas. In summary, there is cell specific regulation of PR by P in the hypothalamus and pituitary wherein P downregulates PR in the pituitary without affecting ER. However, P has no significant effect on PR expression in the hypothalamus even though P decreases ER in the VMN. Although these observations suggest diverse cell specific regulatory mechanisms, they are consistent with ER and PR mediated physiological events such as prolactin secretion and sexual behavior.