OVARIAN STEROID REGULATION OF ESTROGEN & PROGESTIN RECEPTOR MRNA IN HYPOTHALAMUS
OVARIAN STEROID REGULATION OF ESTROGEN & PROGESTIN RECEPTOR MRNA IN HYPOTHALAMUS
批准号:
6247204
负责人:
CYNTHIA Louise BETHEA
金额:
$4.86万
依托单位国家:
美国
项目类别:
财政年份:
1997
资助国家:
美国
项目状态:
已结题
起止时间:
1997-05-01 至 1998-04-30
中文摘要
雌激素(ER)和孕激素受体(PR)mRNA的调控
和蛋白质的同源激素在下丘脑中进行了检测。
类固醇治疗的猴子的脑下垂体。猕猴(Macaca
Mulatta)接受了卵巢切除和子宫切除(去势)和
植入含有雌二醇17(E)或
孕酮(P)。类固醇对雌激素受体和孕激素受体基因表达的调节
用原位杂交法检测下丘脑和垂体
(差不多)。在下丘脑中,ER和PR免疫检测蛋白
还在附近的路段进行了检查。在脑下垂体中,mRNA水平是
与以往相同处理的ER和PR蛋白分析比较
动物。E处理诱导下丘脑内侧基底核PR基因表达
和脑下垂体。补磷处理对PR基因的表达没有影响
在下丘脑中的表达,但显著降低了小鼠脑内PR的表达。
脑下垂体。与PR蛋白检测有很好的一致性
免疫细胞化学(ICC)。E处理组大鼠ER基因表达无明显变化。
下丘脑或脑下垂体。补充磷降低内质网
在腹内侧核(VMN)有表达,但在弓状核不表达
或者在脑下垂体部。ER基因的表达与ER的表达一致
这些区域的蛋白质。总而言之,存在细胞特有的调节
P在下丘脑和垂体对PR的作用,其中P下调
在不影响ER的情况下,垂体PR。然而,P没有
对下丘脑PR表达有显著影响
降低VMN中的ER。尽管这些观察结果表明
细胞特有的调节机制,它们与内质网和
PR介导的生理事件,如催乳素分泌和
性行为。
英文摘要
The regulation of estrogen (ER) and progestin receptor (PR) mRNA
and protein by their cognate hormones was examined in the hypothalamus
and pituitary of steroid treated monkeys. Rhesus macaques (Macaca
mulatta) were ovariectomized and hysterectomized (spayed) and
implanted with Silastic capsules containing estradiol 17 (E) or
progesterone (P). Steroid regulation of ER and PR mRNA levels in the
hypothalamus and pituitary was examined with in situ hybridization
(ISH). In the hypothalamus, ER and PR immunodetectable proteins were
also examined in nearby sections. In the pituitary, mRNA levels were
compared to previous ER and PR protein analysis of identically treated
animals. E treatment induced PR mRNA in the medial basal hypothalamus
and pituitary. Supplemental P treatment had no effect on PR mRNA
levels in the hypothalamus, but markedly reduced PR mRNA in the
pituitary. There was excellent agreement with PR protein detection by
immunocytochemistry (ICC). E treatment had no effect on ER mRNA in
the hypothalamus or pituitary. Supplemental P treatment decreased ER
mRNA in the ventromedial nucleus (VMN), but not in the arcuate nucleus
or in the pituitary. There was agreement between ER mRNA and ER
protein in these areas. In summary, there is cell specific regulation
of PR by P in the hypothalamus and pituitary wherein P downregulates
PR in the pituitary without affecting ER. However, P has no
significant effect on PR expression in the hypothalamus even though P
decreases ER in the VMN. Although these observations suggest diverse
cell specific regulatory mechanisms, they are consistent with ER and
PR mediated physiological events such as prolactin secretion and
sexual behavior.
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会议论文
Postmenopausal Monkey Resource
-
批准号:9104295
-
项目类别:
-
资助金额:$25.63万
-
财政年份:2012
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Postmenopausal Monkey Resource
-
批准号:8337472
-
项目类别:
-
资助金额:$84.76万
-
财政年份:2012
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Postmenopausal Monkey Resource
-
批准号:8705065
-
项目类别:
-
资助金额:$53.46万
-
财政年份:2012
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Postmenopausal Monkey Resource
-
批准号:8532068
-
项目类别:
-
资助金额:$48.85万
-
财政年份:2012
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
OVARIAN STEROID REGULATION OF SEROTONIN NEURAL FUNCTION
-
批准号:8357731
-
项目类别:
-
资助金额:$5.82万
-
财政年份:2011
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
ROLE OF SEROTONIN IN MEDIATING STRESS-INDUCED INFERTILITY
-
批准号:8357841
-
项目类别:
-
资助金额:$5.82万
-
财政年份:2011
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
OVARIAN STEROID REGULATION OF SEROTONIN NEURAL FUNCTION
-
批准号:8357840
-
项目类别:
-
资助金额:$5.82万
-
财政年份:2011
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
IN VITRO MODEL DEVELOPMENT OF SEROTONIN NEURONS
-
批准号:8357741
-
项目类别:
-
资助金额:$3.63万
-
财政年份:2011
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
NOREPINEPHRINE IN STRESS-INDUCED AMENORRHEA OF MACAQUES
-
批准号:8357842
-
项目类别:
-
资助金额:$5.82万
-
财政年份:2011
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
TISSUE BANK
-
批准号:8125604
-
项目类别:
-
资助金额:$2.78万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
IN VITRO MODEL DEVELOPMENT OF SEROTONIN NEURONS
-
批准号:8173186
-
项目类别:
-
资助金额:$4.76万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Steroid Regulation of Serotonin in Males
-
批准号:8241173
-
项目类别:
-
资助金额:$32.37万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Steroid Regulation of Serotonin in Males
-
批准号:8403735
-
项目类别:
-
资助金额:$30.96万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Steroid Regulation of Serotonin in Males
-
批准号:7889423
-
项目类别:
-
资助金额:$49.71万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Norepinephrine in Stress-Induced Amenorrhea of Macaques
-
批准号:8116585
-
项目类别:
-
资助金额:$25.2万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Steroid Regulation of Serotonin in Males
-
批准号:8604749
-
项目类别:
-
资助金额:$32.14万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
OVARIAN STEROID REGULATION OF SEROTONIN NEURAL FUNCTION
-
批准号:8173177
-
项目类别:
-
资助金额:$7.61万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Norepinephrine in Stress-Induced Amenorrhea of Macaques
-
批准号:7771494
-
项目类别:
-
资助金额:$21.78万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Steroid Regulation of Serotonin in Males
-
批准号:8079556
-
项目类别:
-
资助金额:$33.46万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
IN VITRO MODEL DEVELOPMENT OF SEROTONIN NEURONS
-
批准号:7958417
-
项目类别:
-
资助金额:$8.03万
-
财政年份:2009
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
海外基金