OVARIAN STEROID REGULATION OF ESTROGEN & PROGESTIN RECEPTOR MRNA IN HYPOTHALAMUS

雌激素的卵巢类固醇调节

• 批准号: 6247204
• 负责人: CYNTHIA Louise BETHEA
• 金额: $ 4.86万
• 依托单位: OREGON REGIONAL PRIMATE RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 1998-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6247204
• 关键词: Mammalia; Primates; animal tissue; endocrine gland /system; hormones; mental disorders; mother /infant health care; nervous system; reproductive system; women's health

The regulation of estrogen (ER) and progestin receptor (PR) mRNA and protein by their cognate hormones was examined in the hypothalamus and pituitary of steroid treated monkeys. Rhesus macaques (Macaca mulatta) were ovariectomized and hysterectomized (spayed) and implanted with Silastic capsules containing estradiol 17 (E) or progesterone (P). Steroid regulation of ER and PR mRNA levels in the hypothalamus and pituitary was examined with in situ hybridization (ISH). In the hypothalamus, ER and PR immunodetectable proteins were also examined in nearby sections. In the pituitary, mRNA levels were compared to previous ER and PR protein analysis of identically treated animals. E treatment induced PR mRNA in the medial basal hypothalamus and pituitary. Supplemental P treatment had no effect on PR mRNA levels in the hypothalamus, but markedly reduced PR mRNA in the pituitary. There was excellent agreement with PR protein detection by immunocytochemistry (ICC). E treatment had no effect on ER mRNA in the hypothalamus or pituitary. Supplemental P treatment decreased ER mRNA in the ventromedial nucleus (VMN), but not in the arcuate nucleus or in the pituitary. There was agreement between ER mRNA and ER protein in these areas. In summary, there is cell specific regulation of PR by P in the hypothalamus and pituitary wherein P downregulates PR in the pituitary without affecting ER. However, P has no significant effect on PR expression in the hypothalamus even though P decreases ER in the VMN. Although these observations suggest diverse cell specific regulatory mechanisms, they are consistent with ER and PR mediated physiological events such as prolactin secretion and sexual behavior.

雌激素(ER)和孕激素受体(PR)mRNA的调控 和蛋白质的同源激素在下丘脑中进行了检测。 类固醇治疗的猴子的脑下垂体。猕猴(Macaca Mulatta)接受了卵巢切除和子宫切除(去势)和 植入含有雌二醇17(E)或 孕酮(P)。类固醇对雌激素受体和孕激素受体基因表达的调节 用原位杂交法检测下丘脑和垂体 (差不多)。在下丘脑中，ER和PR免疫检测蛋白 还在附近的路段进行了检查。在脑下垂体中，mRNA水平是 与以往相同处理的ER和PR蛋白分析比较 动物。E处理诱导下丘脑内侧基底核PR基因表达 和脑下垂体。补磷处理对PR基因的表达没有影响 在下丘脑中的表达，但显著降低了小鼠脑内PR的表达。 脑下垂体。与PR蛋白检测有很好的一致性 免疫细胞化学(ICC)。E处理组大鼠ER基因表达无明显变化。 下丘脑或脑下垂体。补充磷降低内质网 在腹内侧核(VMN)有表达，但在弓状核不表达 或者在脑下垂体部。ER基因的表达与ER的表达一致 这些区域的蛋白质。总而言之，存在细胞特有的调节 P在下丘脑和垂体对PR的作用，其中P下调 在不影响ER的情况下，垂体PR。然而，P没有 对下丘脑PR表达有显著影响 降低VMN中的ER。尽管这些观察结果表明 细胞特有的调节机制，它们与内质网和 PR介导的生理事件，如催乳素分泌和 性行为。