OVARIAN STEROID REGULATION OF ESTROGEN & PROGESTIN RECEPTOR MRNA IN HYPOTHALAMUS
OVARIAN STEROID REGULATION OF ESTROGEN & PROGESTIN RECEPTOR MRNA IN HYPOTHALAMUS
批准号:
6247204
负责人:
CYNTHIA Louise BETHEA
金额:
$4.86万
依托单位国家:
美国
项目类别:
财政年份:
1997
资助国家:
美国
项目状态:
已结题
起止时间:
1997-05-01 至 1998-04-30
中文摘要
雌激素(ER)和孕激素受体(PR)mRNA的调节
在下丘脑中检查了它们的同源激素和蛋白质
和脑垂体的变化。 恒河猴(Macaca
mulatta)被切除卵巢和卵巢切除(切除卵巢),
植入含雌二醇17(E)的硅橡胶胶囊,或
孕酮(P)。 雌激素对乳腺癌雌激素受体和孕激素受体mRNA水平的调节
用原位杂交技术检测下丘脑和垂体
(ISH)。 在下丘脑中,ER和PR免疫检测蛋白是
在附近的部分也进行了检查。 在垂体中,mRNA水平是
与之前相同处理的ER和PR蛋白分析相比,
动物 E处理诱导下丘脑内侧基底核PR mRNA表达
和垂体。 补磷处理对PR mRNA表达无影响
水平,但明显减少PR mRNA在下丘脑,
脑垂体 与PR蛋白检测结果有很好的一致性,
免疫细胞化学(ICC)。 E处理对ER mRNA表达无影响。
下丘脑或脑垂体。 补充磷处理降低ER
mRNA在腹内侧核(VMN)中表达,而不在弓状核中表达
或脑垂体中。 ER mRNA表达与ER
蛋白质在这些领域 总之,存在细胞特异性调节
下丘脑和垂体中P下调PR,
垂体PR而不影响ER。 P没有
显著影响PR在下丘脑的表达,即使P
降低VMN中的ER。 尽管这些观察表明,
细胞特异性调节机制,它们与ER一致,
PR介导的生理事件,如催乳素分泌和
性行为
英文摘要
The regulation of estrogen (ER) and progestin receptor (PR) mRNA
and protein by their cognate hormones was examined in the hypothalamus
and pituitary of steroid treated monkeys. Rhesus macaques (Macaca
mulatta) were ovariectomized and hysterectomized (spayed) and
implanted with Silastic capsules containing estradiol 17 (E) or
progesterone (P). Steroid regulation of ER and PR mRNA levels in the
hypothalamus and pituitary was examined with in situ hybridization
(ISH). In the hypothalamus, ER and PR immunodetectable proteins were
also examined in nearby sections. In the pituitary, mRNA levels were
compared to previous ER and PR protein analysis of identically treated
animals. E treatment induced PR mRNA in the medial basal hypothalamus
and pituitary. Supplemental P treatment had no effect on PR mRNA
levels in the hypothalamus, but markedly reduced PR mRNA in the
pituitary. There was excellent agreement with PR protein detection by
immunocytochemistry (ICC). E treatment had no effect on ER mRNA in
the hypothalamus or pituitary. Supplemental P treatment decreased ER
mRNA in the ventromedial nucleus (VMN), but not in the arcuate nucleus
or in the pituitary. There was agreement between ER mRNA and ER
protein in these areas. In summary, there is cell specific regulation
of PR by P in the hypothalamus and pituitary wherein P downregulates
PR in the pituitary without affecting ER. However, P has no
significant effect on PR expression in the hypothalamus even though P
decreases ER in the VMN. Although these observations suggest diverse
cell specific regulatory mechanisms, they are consistent with ER and
PR mediated physiological events such as prolactin secretion and
sexual behavior.
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Postmenopausal Monkey Resource
-
批准号:9104295
-
项目类别:
-
资助金额:$25.63万
-
财政年份:2012
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Postmenopausal Monkey Resource
-
批准号:8337472
-
项目类别:
-
资助金额:$84.76万
-
财政年份:2012
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Postmenopausal Monkey Resource
-
批准号:8705065
-
项目类别:
-
资助金额:$53.46万
-
财政年份:2012
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Postmenopausal Monkey Resource
-
批准号:8532068
-
项目类别:
-
资助金额:$48.85万
-
财政年份:2012
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
OVARIAN STEROID REGULATION OF SEROTONIN NEURAL FUNCTION
-
批准号:8357731
-
项目类别:
-
资助金额:$5.82万
-
财政年份:2011
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
ROLE OF SEROTONIN IN MEDIATING STRESS-INDUCED INFERTILITY
-
批准号:8357841
-
项目类别:
-
资助金额:$5.82万
-
财政年份:2011
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
OVARIAN STEROID REGULATION OF SEROTONIN NEURAL FUNCTION
-
批准号:8357840
-
项目类别:
-
资助金额:$5.82万
-
财政年份:2011
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
IN VITRO MODEL DEVELOPMENT OF SEROTONIN NEURONS
-
批准号:8357741
-
项目类别:
-
资助金额:$3.63万
-
财政年份:2011
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
NOREPINEPHRINE IN STRESS-INDUCED AMENORRHEA OF MACAQUES
-
批准号:8357842
-
项目类别:
-
资助金额:$5.82万
-
财政年份:2011
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
TISSUE BANK
-
批准号:8125604
-
项目类别:
-
资助金额:$2.78万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
IN VITRO MODEL DEVELOPMENT OF SEROTONIN NEURONS
-
批准号:8173186
-
项目类别:
-
资助金额:$4.76万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Steroid Regulation of Serotonin in Males
-
批准号:8241173
-
项目类别:
-
资助金额:$32.37万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Steroid Regulation of Serotonin in Males
-
批准号:8403735
-
项目类别:
-
资助金额:$30.96万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Steroid Regulation of Serotonin in Males
-
批准号:7889423
-
项目类别:
-
资助金额:$49.71万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Norepinephrine in Stress-Induced Amenorrhea of Macaques
-
批准号:8116585
-
项目类别:
-
资助金额:$25.2万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Steroid Regulation of Serotonin in Males
-
批准号:8604749
-
项目类别:
-
资助金额:$32.14万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
OVARIAN STEROID REGULATION OF SEROTONIN NEURAL FUNCTION
-
批准号:8173177
-
项目类别:
-
资助金额:$7.61万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Norepinephrine in Stress-Induced Amenorrhea of Macaques
-
批准号:7771494
-
项目类别:
-
资助金额:$21.78万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
Steroid Regulation of Serotonin in Males
-
批准号:8079556
-
项目类别:
-
资助金额:$33.46万
-
财政年份:2010
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
IN VITRO MODEL DEVELOPMENT OF SEROTONIN NEURONS
-
批准号:7958417
-
项目类别:
-
资助金额:$8.03万
-
财政年份:2009
-
负责人:CYNTHIA Louise BETHEA
-
依托单位:
海外基金