CORE--IMMUNOHISTOCHEMISTRY

组织切片上抗原的免疫组织化学鉴定 成为现代组织病理学不可或缺的有价值的一部分。抗原 通过免疫荧光技术或酶定位- 包埋或冷冻材料的衬底方法。尽管这些 方法常规为软组织标本，免疫组织化学 对骨关节组织的检查因 骨和软骨的材料特性。特殊的技术是 需要处理未脱钙的骨以进行免疫组织化学，例如 使用水溶性、冷聚合塑料或使用 冷冻薄片后的特殊粘合剂、支撑物和载玻片 电动低温恒温器上的材料。通过这些技术，人们可以识别 存在于细胞表面的抗原，无论是炎症性的 软骨、骨、滑膜或任何其他组织中的浸润物； 基质中存在非细胞抗原。该计划的目标是 俄亥俄州东北部多用途关节炎的免疫组织化学核心 中心将继续作为共享资源发挥作用，并集中 用于生产与以下有关的单抗的设施 肌肉骨骼研究以及加工、嵌入、切割和 骨、软骨和关节相关的免疫组织化学染色 作为中心正在进行的研究活动的一部分生成的样本 人事部。免疫组织化学实验室的集中化 提供经济实惠的用品、设备和工作；提供新的 向目前未使用这些技术的调查人员提供技术；以及 促进NEOMAC中不同调查人员之间的互动。

The immunohistochemical identification of antigens on tissue sections has become an integral and valuable part of modern histopathology. Antigens are localized either by immunofluorescent techniques or by enzyme- substrate methods on embedded or frozen materials. Although these methods are routine for soft tissue samples, immunohistochemical examination of osteocartilaginous tissues is made difficult by the material properties of bone and cartilage. Special techniques are required to process nondecalcified bone for immunohistochemistry such as the use of water soluble, cold polymerizing plastics or the use of special adhesives, supports, and slides after thin sectioning of frozen material on a motorized cryostat. With these techniques one can identify antigens present on the surface of cells, whether in inflammatory infiltrates, in cartilage, bone, synovium, or any other tissue; and noncellular antigens present within the matrix. The objective of the Immunohistochemistry Core of the northeast ohio multipurpose Arthritis Center is to continue to function as a shared resource and centralized facility for the production of monoclonal antibodies relevant to musculoskeletal research and for the processing, embedding, cutting, and immunohistochemical staining of bone, cartilage, and joint-related samples generated as part of on-going research activities of center personnel. The centralization of the immunohistochemistry laboratory provides economy of supplies, equipment, and effort; offers new technology to investigators not currently using these techniques; and promotes interactions among the various investigators in the NEOMAC.