HIGH PRESSURE DIFFERENTIAL SCANNING CALORIMETER

压力提供了一个额外的热力学变量， 大分子稳定性研究。 压力扰动将 取代大分子的构象平衡， 每种构象的相对体积。 压力不能 仅影响蛋白质的稳定性而不引入 化学变量，但也能够稳定选择部分 折叠构象 因此，它应该成为重要的 用于研究蛋白质稳定性和折叠机制的仪器。 差示扫描量热仪的操作能力， 在不同的恒定压力下， 生物量热中心 在本年度，初始 已开始进行可行性研究。 可以想象， 发展将分两个阶段进行。 在第一阶段中，DSC具有 一个能够承受高达500个大气压的加固单元将被 开发 在第二阶段，一个热量计，其中内部 以及电池的外部被加压 设计了 这第二台仪器将能够维持 压力接近4,000大气压

Pressure provides an additional thermodynamic variable for the study of macromolecular stability. A pressure perturbation will displace the conformational equilibrium of a macromolecule according to the relative volume of each conformation. Pressure is capable not only of affecting the stability of a protein without introducing a chemical variable, but is also able to stabilize selected partially folded conformations. As such, it should become an important instrument for the study of protein stability and folding mechanisms. A differential scanning calorimeter with the capability of operating at different constant pressures represent a long term design plan for the Biocalorimetry Center. During the current year, initial feasibility studies were initiated. It is visualized that this development will be made in two stages. In the first stage a DSC with reinforced cell capable of sustaining up to ~500 atm will be developed. In the second phase, a calorimeter in which the interior as well as the exterior of the cell are pressurized will be designed. This second instrument will be capable of sustaining pressures approaching 4,000 atm