CORE--CELL DISTRIBUTION/CHARACTERIZATION

核心——细胞分布/表征

• 批准号: 6101971
• 负责人: MARY K DANKS
• 金额: $ 16.71万
• 依托单位: ST. JUDE CHILDREN'S RESEARCH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6101971
• 关键词: Adenoviridae; biomedical facility; cell line; clone cells; cytogenetics; gene expression; neoplastic cell; neuroblastoma; oncogenes; pediatric neoplasm /cancer; restriction fragment length polymorphism; rhabdomyosarcoma; tissue /cell culture; tumor suppressor genes; xenotransplantation

The purposes of the Cell Line Characterization Core facility are: 1) To provide investigators of the STPPG with well-characterized cell lines derived from solid tumors of pediatric patients; 2) to establish, on a limited basis, cell lines that are not currently available from tumors of pediatric patients; 3) to maintain a data base that will include, but will not be limited to, oncogene and tumor suppressor genes expressed by each cell line and DNA fingerprinting for consistent identification of each cell line; 4) To provide bulk culture for establishment of xenografts; and 5) To amplify and provide characterized adenovirus to in vitro and in vivo use in the outlined projects.

细胞系表征核心机构的目的是： 第一章 为STPPG的研究者提供充分表征的细胞 来源于儿科患者实体瘤的细胞系; 2)在有限的基础上，建立目前还没有的细胞系， 可从儿科患者的肿瘤中获得; 3)维护数据库，包括但不限于 各细胞系表达的癌基因和肿瘤抑制基因， DNA指纹分析，用于一致地鉴定每个细胞系; 4)为建立异种移植物提供大量培养物;以及 5)目的：体外扩增腺病毒，并提供特征性腺病毒， 在概述的项目中使用活体。