MUTANT GENES IN FECES FOR EARLY DETECTION OF COLORECTAL NEOPLASMS

结直肠癌是美国癌症死亡的第二大原因。 各州。1996年，将发生约133,500起新病例， 大约有55,000人将死于这种疾病。几项研究 已经表明，筛查无症状人群的大肠肿瘤 可以降低死亡率。分子遗传学研究表明， Ras原癌基因的突变激活和突变失活 许多抑癌基因与结直肠肿瘤的发生有关。这个 这项建议的目的是探索早期诊断的遗传方法。 结直肠癌的检测。我们力求实现以下目标： 1.建立可靠的检测粪便中ras基因突变的方法 样本，以努力提供单一样本筛选测试 结直肠癌。通过以下方法评估检测的敏感性和特异性 既往受试者粪便标本的回顾性分析 有确证的癌症或腺瘤。 2.检测粪便ras癌基因突变的遗传学方法比较 对粪便潜血进行免疫化学检测的一项前瞻性研究 在接受结肠镜检查的患者中。

Colorectal cancer is the second leading cause of cancer death in the United States. In 1996, approximately 133,500 new cases will occur and approximately 55,000 persons will die from this disease. Several studies have shown that screening asymptomatic populations for colorectal neoplasia can reduce mortality. Molecular genetic studies have demonstrated that the mutational activation of ras proto-oncogenes and mutational inactivation of tumor suppressor genes are associated with colorectal tumorigenesis. The objective of this proposal is to explore genetic approaches for early detection of colorectal cancer. We seek to achieve the following: 1. Generate a robust method for detecting ras gene mutations in stool specimens in an effort to provide a single specimen screening test for colorectal cancer. Evaluate test sensitivity and specificity through retrospective analysis of stool samples previously obtained from subjects with documented carcinomas or adenomas. 2. Compare the genetic method for detecting fecal ras oncogene mutation with an immunochemical test for fecal occult blood in a prospective study in patients undergoing surveillance colonoscopy.