MUTANT GENES IN FECES FOR EARLY DETECTION OF COLORECTAL NEOPLASMS

结直肠癌是美国癌症死亡的第二大原因 国家。 1996年，大约将发生133,500个新病例， 大约55,000人将死于这种疾病。多项研究 研究表明，对无症状人群进行结直肠肿瘤筛查 可以降低死亡率。分子遗传学研究表明， ras原癌基因的突变激活和突变失活 肿瘤抑制基因与结直肠肿瘤的发生有关。的 该提案的目的是探索早期的遗传方法 结肠直肠癌的检测。我们力求实现以下目标： 1. 建立一种稳健的方法来检测粪便中的ras基因突变 样本，努力提供单一样本筛选测试 结直肠癌。通过评估测试的敏感性和特异性 对先前从受试者获得的粪便样本进行回顾性分析 有记录的癌或腺瘤。 2、比较检测粪便ras癌基因突变的遗传学方法 在一项前瞻性研究中对粪便潜血进行免疫化学测试 接受监测结肠镜检查的患者。

Colorectal cancer is the second leading cause of cancer death in the United States. In 1996, approximately 133,500 new cases will occur and approximately 55,000 persons will die from this disease. Several studies have shown that screening asymptomatic populations for colorectal neoplasia can reduce mortality. Molecular genetic studies have demonstrated that the mutational activation of ras proto-oncogenes and mutational inactivation of tumor suppressor genes are associated with colorectal tumorigenesis. The objective of this proposal is to explore genetic approaches for early detection of colorectal cancer. We seek to achieve the following: 1. Generate a robust method for detecting ras gene mutations in stool specimens in an effort to provide a single specimen screening test for colorectal cancer. Evaluate test sensitivity and specificity through retrospective analysis of stool samples previously obtained from subjects with documented carcinomas or adenomas. 2. Compare the genetic method for detecting fecal ras oncogene mutation with an immunochemical test for fecal occult blood in a prospective study in patients undergoing surveillance colonoscopy.