CORE--VIRAL PATHOGENESIS

病毒致病核心作为分离株的集中来源 以及与HIV-1、HIV-2和SIV相关的测定。此外，核心作为 用户与UCSD CFAR成员实验室之间的接口 专门研究影响HIV感染个体的疱疹病毒（CMV， HSV和HHV-8）。提供四种基本类型的服务：（1）作为病毒 资源，核心提供等分试样从广泛的收集， 对HIV-1、HIV-2和SIV的病毒株进行表征和滴定。(2)作为 细胞资源，核心提供病毒易感细胞，包括细胞 系、分离的CD 4 + T细胞和巨噬细胞。(3)如病毒 表征资源，核心提供测试细胞向性， 合胞体诱导（SI）与非SI，共受体使用，细胞病变 与非细胞病变的，复制动力学，药物敏感性，和 艾滋病毒的其他特性(4)作为一种筛选服务，核心提供 对研究性治疗剂（抗病毒化合物， 基因疗法、抗体中和等）。 这些资源使得 对于新手和有经验的调查人员来说， 病毒学的研究思路。如果没有核心，每个调查员都需要 建立和验证所需的不同方法和测定， 工作，这可能需要几个月。此外，许多核心的用户没有BL 3 自己培训或体验，因此需要花费数月的时间 努力获得使用这些病毒所需的许可。而 “核心”计划并不意味着要取代由以下机构建立的全面研究计划： 作为主要项目之一的调查员，它是进行 中试实验和筛选具有抗病毒活性的化合物。

The Viral Pathogenesis Core serves as a centralized source for isolates and assays related to HIV-1, HIV-2, and SIV. In addition, the Core acts as an interface between users and the laboratories of UCSD CFAR members specializing in the herpes virus affecting HIV-infected individuals (CMV, HSV, and HHV-8). Four basic types of services are provided: (1) As a viral resource, the Core provides aliquots from a wide collection of characterized and titered viral strains of HIV-1, HIV-2, and SIV. (2) As a cell resource, the Core provides virus susceptible cells, including cell lines, isolated CD4+ T cells and macrophages. (3) As a virus characterization resource, the Core provides testing for cell tropisms, syncytium-inducing (SI) versus non-SI, co-receptor usage, cytopathic versus non-cytopathic, replication kinetics, drug susceptibilities, and other properties of HIV. (4) As a screening service, the Core provides the initial testing for investigational therapeutics (antiviral compounds, gene therapies, antibody neutralization, etc.). These resources make it easier for both novices and experienced investigators to pursue their virological research ideas. Without the Core, each investigator would need to set up and validate the different methods and assays required for their work, which could take months. Also, many of the Core's users have no BL3 training or experience themselves, and thus would need to expend months of effort on gaining the permission needed to work with these viruses. While the Core is not meant to replace a full scale research program set up by an investigator as one of the major projects, it is ideal for conducting pilot experiments and the screening of compounds for antiviral activity.