CORE--MORPHOLOGY/IMAGING FACILITY

核心——形态学/成像设备

• 批准号: 6270712
• 负责人: ROBERT D SPECIAN
• 金额: $ 13.6万
• 依托单位: LOUISIANA STATE UNIV HSC SHREVEPORT
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6270712
• 关键词: biomedical facility; confocal scanning microscopy; electron microscopy; histology; light microscopy; morphology; tissue /cell culture; tissue /cell preparation

In order to provide core support for the research projects, the Program Project Grant has four Core units, i.e., an Administrative Core (P.I.; D.N.Granger), a Cell Culture Core (P.I.; P.R Kvietys), a Biochemistry Core (P/I/; M.B. Grisham), and a Morphology Core (P.I.; R. Specian). These Cores will provide shared facilities and resources for the investigators in the PPG and play an important role in enhancing the interaction among investigators. The primary functions of the Administrative Core are to co- ordinate the activities of the component Projects and Cores, to manage purchasing and budgetary matters, to provide secretarial support for the various Projects and Cores, and to schedule meetings and seminars in the PPG. The cell Culture Core will provide cultured endothelial and epithelial cells for specific Projects in the PPG. The Biochemistry Core will perform various spectrophotometric and fluorometric assays, protein separation and purification, and enzyme inactivation procedures for several projects. The Morphology Core will provide facilities and expertise for light and electron microscopic studies of the mucosal membrane, blood vessels, leukocytes, and cultured endothelial cells. The four Core units will provide an efficient means for generating the services needed to conduct the proposed research and to attain the stated goals of the Program.

为了为研究项目提供核心支持，该计划 项目赠款有四个核心单位，即行政核心(P.I.； D.N.Granger)，一个细胞培养核心(P.I.；P.R.Kvietys)，一个生化核心 (P/I/；M.B.Grisham)和形态核心(P.I.；R.Specian)。这些 核心将为#年的调查人员提供共享的设施和资源 PPG和在加强 调查人员。行政核心的主要职能是共同- 协调组件项目和核心项目的活动，以管理 采购和预算事务，为 各种项目和核心，并安排会议和研讨会在 PPG。细胞培养核心将为培养的内皮细胞和 PPG中特定项目的上皮细胞。生物化学的核心 将进行各种分光光度和荧光分析，蛋白质 几种酶的分离纯化和酶灭活程序 项目。形态核心将提供设施和专业知识 粘膜、血液的光镜和电子显微镜研究 血管、白细胞和培养的内皮细胞。四大核心单元 将提供一种高效的方法来生成所需的服务 进行拟议的研究，并实现 程序。