DNA AND HYBRID STRUCTURE BY SOLUTION NMR

局部碱基对最近邻上下文效应的系统研究 通过完成基于NMR的解决方案， CGNATNCG系列中剩余2个结构的结构和 CGNTANCG系列中的三个剩余结构。 这5个建议 序列是BamH 1、Sph I、Nhe I、Eco RV和 Kpn I限制性内切核酸酶。 在B '-DNA中使B-DNA成核所需的临界大小的问题 环境将通过研究一系列的双链体的阐明 A5 NnT 5型（其中n在1-4之间变化），以解释凝胶 在寡聚化的N5-iAiTiN 5-i序列中的阻滞弯曲测定。 的 在B-B'连接处和B'-B连接处的弯曲的结构将是 在非对称十二聚体和对称十六聚体中进行了研究， 以便更好地确定侧翼B-DNA片段的螺旋轴。 弯曲的结构和程度也将作为一个函数进行研究 的侧翼序列和G残基的结构效应 将研究插入B ′ oligo-A束的基因。 DNA双链体和杂交体之间共价连接的结构 DNA：RNA双链体将通过NMR进行研究，特别是逆转录病毒连接 序列，以及在右侧长末端重复序列的ds DNA序列， 将研究HIV-1，试图阐明其结构 LTR整合到人类基因组中的能力的基础。 除了这些目标，在特定序列上的合作 将开展其他计划实验室感兴趣的活动， 同位素固态（DRAMA）原子距离测量将是 与Drobny实验室一起进行，以记录结构特性 在我们的高分辨率结构研究中观察到的。

A systematic study of nearest neighbor context effects on local base pair structure will be continued by completing the NMR-based solution structures of the 2 remaining structures in the CGNATNCG series and the three remaining structures in the CGNTANCG series. These 5 proposed sequences are the restriction sites for Bam H1, Sph I, Nhe I, Eco RV and Kpn I restriction endonucleases. The question of the critical size required to nucleate B-DNA in a B'-DNA environment will be elucidated by studying a series of duplexes of the type A5NnT5 (where n is varied from 1-4) in order to interpret gel retardation bending assays in oligomerized N5-iAiTiN5-i sequences. The structure of the bend at B-B' junctions and B'-B junctions will be studied in non-symmetrical dodecamers and in symmetrical hexadecamers in order to better define the helical axis of the flanking B-DNA segments. The structure and extent of bending will also be studied as a function of the flanking context sequence and the structural effects of G residues inserted into the B'oligo-A tract will be investigated. The structure of covalent junctions between DNA duplexes and hybrid DNA:RNA duplexes will be studied by NMR, especially retroviral junction sequences, and the ds DNA sequence at the right long-terminal-repeat of HIV-I will be investigated in an attempt to elucidate the structural basis for the ability of this LTR to integrate into the human genome. In addition to these goals, collaborative work on particular sequences of interest to other program laboratories will be carried out, and isotopic solid-state (DRAMA) atomic distance measurements will be undertaken with the Drobny lab to document structural peculiarities observed in our high-resolution structural studies.