CORE--AUTOMATION AND INSTRUMENTATION

描述：（改编自申请人摘要）本核心 实验室致力于开发新的自动化系统， 基因组作图和测序。 A部分自动化将开发新的 生产部门执行的方法和手段 项目1和2，根据现有方法， 原型机器人 这些发展将包括： 自动化，杂交探针系统，平行寡核苷酸 合成，Biomek的开发，琼脂糖塞制备 系统，注塑塑料制品，先进的菌落挑选系统， 增强循环测序技术和GIST。 该项目将 还研究了纳米体积的操作、检测和分析 为未来的微测序方法提供样本。 B部分将具体说明 致力于改进传统的、市售的 基于荧光的DNA测序，这可能会导致 在吞吐量和效率上。 具体而言，该项目将实施 使用改进的凝胶形式，例如长的、薄的、热稳定的 用于增加读取长度的凝胶，以及用于增加通量的更宽的凝胶。 提出了改进自动化数据采集系统的方法 测序仪通过提供更有效的激发和收集光学器件， 更高增益的光电管，更快的数据采集硬件和软件， 最终，使用四光电管读出系统，并提高 现有的碱基调用软件， 更有用，并建立一个综合数据收集系统， 处理系统，其结合质量控制措施以监测 项目2测序操作中测序仪的性能。

Description: (Adapted from the applicant's abstract) This core laboratory is devoted to the development of new automation systems for genome mapping and sequencing. Part A, automation, will develop new methods and instruments for implementation in the production sections of Projects 1 and 2, based upon currently available methodology and prototype robotics. These developments will include: contig assembly automation, a hybridization probing system, parallel oligonucleotide synthesis, developments for the Biomek, an agarose plug preparation system, injection molded plasticware, an advanced colony picking system, technology for enhanced cycle sequencing, and the GIST. The project will also investigate the manipulation, detection and analysis of nanovolume samples for future microsequencing approaches. Part B will specifically address improvements to conventional, commercially available fluorescence-based DNA sequencing, which could lead to major increases in throughput and efficiency. Specifically, this project will implement the use of improved gel formats, such as long, thin, thermally stabilized gels for increased read lengths, and wider gels to increase throughput. It is proposed to improve the data acquisition system of the automated sequencers by providing more efficient excitation and collection optics, higher gain phototubes, faster data acquisition hardware and software and ultimately, the use of a four phototube readout system, and to improve the existing base-calling software so that the sequence data generated is more useful and to develop an integrated data collection and processing system incorporating a quality control measure to monitor the performance of the sequencers in the sequencing operation of Project 2.