ISOLATION AND SEROTYPIC CHARACTERIZATION OF HUMAN AND ANIMAL ROTAVIRUSES

人和动物轮状病毒的分离和血清型特征

• 批准号: 6098912
• 负责人: Yasutaka Hoshino
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6098912
• 关键词: Rotavirus; antibody specificity; antiviral antibody; capsid; epidemiology; gene rearrangement; genetic strain; microorganism culture; microorganism immunology; neutralizing antibody; nucleic acid sequence; serotyping; tissue /cell culture; virus antigen; virus genetics; virus protein

In this project we determined antigenic relationships based on VP4 and VP7 neutralization specificities of various rotavirus strains derived from humans and animals. The elucidation of the neutralization specificities of rotavirus is important in order to achieve a more comprehensive understanding of rotavirus epidemiology and for formulation of an effective strategy for vaccination. By using various single gene substitution human x human or human x bovine rotavirus reassortants and hyperimmune guinea pig antiserum raised against each reassortant, we successfully characterized the VP4 neutralization specificity of representative human rotavirus strains (Wa, P, W161, DS-1, ST3, K8, 69M, Ro1845, PA169, and HAL1166) and established seven distinct VP4 (P) serotypes based on a <20-fold antibody difference criterion that is applied to classification of VP7 serotypes. The VP4 of vervet monkey rotavirus SA11 which has been used by many investigators as a prototype strain of group A rotaviruses was found to belong to serotype 5B which is represented by rhesus monkey rotavirus MMU18006 VP4. The US1205 strain (G serotype 9) which was originally isolated from a child with diarrhea in the U.S. was shown to carry VP4 serotype 2 (prototype strain ST3).

在这个项目中，我们确定了抗原关系 基于不同的VP 4和VP 7中和特异性， 来源于人和动物的轮状病毒株。阐明 中和轮状病毒的特异性是重要的， 为了更全面地了解轮状病毒， 流行病学和制定有效的战略， 预防针通过使用各种单基因置换人类x 人或人X牛轮状病毒抑制剂和超免疫 豚鼠抗血清对每一个resistant，我们 成功表征了 代表性的人轮状病毒株（Wa，P，W161，DS-1，ST 3， K8，69 M，Ro 1845，PA 169和HAL 1166），并建立了7个 基于<20倍抗体差异的不同VP 4（P）血清型 适用于VP 7血清型分类的标准。的vp 4 轮状病毒SA 11，它已经被许多人使用， 研究人员作为A组轮状病毒的原型菌株， 发现属于以恒河猴为代表的血清型5 B 猴轮状病毒MMU 18006 VP 4. US 1205菌株（G 血清型9），其最初从患有腹泻的儿童分离 在美国，显示携带VP 4血清型2（原型菌株 ST 3）。