MOLECULAR ANALYSIS OF NEUTROPHIL ACTIVATION BY CHEMOATTRACTANTS

当这个项目开始时，它的目的是 确定调控吞噬细胞迁移到 感染和炎症部位，重点放在最初的 已知细胞与细胞外信号分子的相互作用 作为化学诱导剂。吞噬细胞化学诱导剂的例子 包括细菌衍生的N-甲酰肽， 补体衍生多肽C5a和一大类结构 被称为趋化因子的相关多肽。最近，这个项目 已经扩展到包括淋巴细胞趋化受体。 此外，我们还发现各种微生物病原体， 包括艾滋病毒在内，利用化学吸引剂受体作为其 生命周期。因此，我们的具体目标也扩大到 包括以下内容：(一)确定DISTINCT的结构 化学诱导剂受体；(Ii)测定化学物质 化学诱导剂的激活和调节机制 受体；(Iii)描绘信号转导途径 被化学引诱剂激活；(Iv)测定一般和 每种趋化受体的特殊生物学作用；以及 (V)描述生物作用和作用机制 微生物致病机制中的趋化受体。

When this project originated, its purpose was to determine the precise factors regulating phagocyte migration to sites of infection and inflammation, focusing on the initial interaction of the cell with extracellular signaling molecules known as chemoattractants. Examples of phagocyte chemoattractants include bacterially-derived N-formyl peptides, the complement-derived peptide C5a, and a large class of structurally related peptides known as chemokines. More recently, this project has expanded to include lymphocyte chemoattractant receptors. Moreover, we have discovered that various microbial pathogens, including HIV, exploit chemoattractant receptors as part of their life cycle. Thus, our specific objectives have also expanded to include the following: (i) to determine the structure of distinct chemoattractant receptors; (ii) to determine the chemical mechanism of activation and regulation of chemoattractant receptors; (iii) to delineate the signal transduction pathways activated by chemoattractants; (iv) to determine the general and specialized biological roles of each chemoattractant receptor; and (v) to delineate the biological role and the mechanism of action of chemoattractant receptors in microbial pathogenesis.