STRUCTURAL ANALYSIS OF BARLEY YELLOW DWARF VIRUS

大麦黄矮病毒的结构分析

• 批准号: 2757545
• 金额: $ 0.05万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2757545
• 关键词: biomedical resource; biotechnology; communicable diseases; microscopy; plants

We are studying the structure of the barley yellow dwarf (BYDV) virus. This virus infects and is limited to the phloem tissues of grasses. The viruses are obligately transmitted by aphids in a strain-specific and persistent-circulative manner. Virus particles contain two proteins, a major 22 kDa protein and smaller amounts of a 72kDa protein. The 72kDa protein is produced by read through of the termination codon of the gene encoding the 22kDa protein. The read-through domain has recently been shown to be present on the surface of virus particles where it may form projections which could interact with membrane bound receptors in the aphid vectors. We p!an to use the cryo-HRSEM techniques that were developed at the IMR for the study of the reo virus.

我们正在研究大麦黄矮病的结构。 病毒。这种病毒侵染并仅限于植物的韧皮部组织。 青草。这些病毒是由蚜虫在一种 菌株特异性和持续循环方式。病毒颗粒 含有两种蛋白质，一种主要的22 kDa蛋白质和少量的 72 kDa蛋白。72 kDa蛋白质是通过通读 编码22 kDa蛋白的基因的终止密码子。这个 直读域最近被证明存在于 病毒颗粒的表面，在那里它可能形成突起， 与蚜虫载体中的膜结合受体相互作用。我们在一起！ 为了使用在IMR开发的冷冻-HRSEM技术 对雷奥病毒的研究。