LOCALIZATION OF NADH GLUTAMATE SYNTHASE IN ALFALFA ROOT NODULES

NADH 谷氨酸合成酶在苜蓿根瘤中的定位

• 批准号: 2757561
• 金额: $ 0.05万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2757561
• 关键词: biomedical resource; enzymes; immunology; microscopy; plants

alfalfa root nodules have the capability to fix atmospheric dinitrogen through symbiosis with the soil born bacteria Rhizobium meliloti. Many enzymes are involved in the assimilation of symbiotically fixed nitrogen. One is the NADH-dependent Glutamate Synthase (NADH-GOGAT) which forms two Glutamate out of Glutamine and a-oxoglutarate. The enzyme is thought to be the rate limiting step in this pathway. In order to understand the biochemistry of the nitrogen assimilation in root nodules, localization of NADH-GOGAT is crucial. We will high-pressure freeze samples of root nodules and then freeze substitute, embed and sectioned. Sections will be immunostained and examined in the TEM in order to localize NADH-GOGAT.

苜蓿根瘤具有固定大气的能力， 通过与土壤中的根瘤菌共生， 苜蓿草。 许多酶都参与了同化 共生固氮 一种是依赖于NADH的谷氨酸 合酶（NADH-GOGAT），从谷氨酰胺和谷氨酸盐中形成两个谷氨酸盐， α-酮戊二酸盐。 这种酶被认为是 这条路。 为了了解氮的生物化学 在根瘤的同化中，NADH-GOGAT的定位是至关重要的。 我们将高压冷冻根瘤样品， 替代、嵌入和分段。 将对切片进行免疫染色， 在TEM中检查以定位NADH-GOGAT。