CONTEMPORARY APPROACHES IN MOLECULAR/STRUCTURAL BIOLOGY

分子/结构生物学的当代方法

• 批准号: 2772042
• 负责人: Daniel T. Simmons
• 金额: $ 30.2万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-30 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2772042
• 关键词: biomedical facility; health science research support; monoclonal antibody; structural biology; tissue /cell culture; transfection /expression vector

The University of Delaware is initiating a major effort and committing significant resources into molecular and structural biology on campus. An external search for a new molecular biologist as Chair of Biological Sciences is presently underway and funds have been committed to hire a number of new faculty in molecular/structural biology over the next few years. The focus of the present IdeA grant complements well with our major thrust Directly related to this application, we are proposing to recruit three molecular biology faculty doing research on a) cell signaling, b) developmental, cell cycle, or cancer biology using transgenic or knock-out mouse technology, and c) gene delivery and targeting. In addition, we are proposing to hire an X-ray crystallographer who is interested in protein- nucleic acid interactions. To recruit the best possible candidates for these positions, we are planning to develop a molecular cell biology facility and an X-ray crystallography laboratory. Once in place, the facilities will be of enormous benefit to the new hires and to existing faculty working on health-related problems. The cell biology facility will consist of three major parts: a) a cell culture laboratory involved in the growth and maintenance of various cell types including embryonal stem cells that will be used for the generation of transgenic and/or knock-out mice, b) an expression and vector facility to be used for introducing genes into cells, sequencing DNA, and facilitating the production of cloned gene products, and c) a monoclonal antibody and immunochemistry facility that will generate immunological reagents and study cells by confocal microscopy to determine the distribution of antigen or to obtain structural and functional information The X-ray crystallography center will be a modem facility directed by a new faculty member to determine the three dimensional structure of important biomolecules. We envision that the new hires and the added facilities will result in a number of new NIH- funded projects at Delaware by the end of the funding period for this grant.

特拉华州大学正在发起一项重大努力， 分子和结构生物学的重要资源。一个 外部寻找一位新的分子生物学家担任生物学系主任 科学目前正在进行中，资金已承诺聘请一名 在未来几年内，分子/结构生物学的新教师人数 年目前IdeA赠款的重点与我们的专业互补 与此应用程序直接相关，我们建议招聘 三个分子生物学系研究a）细胞信号，B） 使用转基因或敲除的发育、细胞周期或癌症生物学 小鼠技术，和c）基因递送和靶向。此外，我们在 提议雇佣一个对蛋白质感兴趣的X射线晶体学家 核酸相互作用招募最好的候选人 这些职位，我们正计划发展一个分子细胞生物学 实验室和X射线晶体学实验室。一旦到位， 设施将为新员工和现有员工带来巨大好处 致力于解决健康相关问题的教师。细胞生物学设施将 由三个主要部分组成：a）细胞培养实验室， 包括胚胎干细胞在内的各种细胞类型的生长和维持 将用于产生转基因和/或敲除 小鼠，B）用于引入的表达和载体设施 基因进入细胞，测序DNA，并促进生产 克隆的基因产物，和c）单克隆抗体和免疫化学 该设施将产生免疫试剂和研究细胞， 共聚焦显微镜以确定抗原的分布或获得 结构和功能信息X射线晶体学中心 将是一个调制解调器设施由一个新的教员，以确定 重要生物分子的三维结构。我们设想 新的招聘和增加的设施将导致一些新的国家卫生研究院- 在特拉华州的资助项目结束时， 格兰特.