GENETIC PREDISPOSITION TO SECOND MALIGNANT NEOPLASMS FOLLOWING CHILDHOOD

这项研究现在是公开的，到目前为止，来自儿童基金会的38名参与者 费城医院已被录取。同意书已经被 签了名。从这些患者那里获得了完整的家族史。 我期待着采集血样进行基因测试 在下个月内开始。 其他第二种恶性肿瘤病例和适当的控制措施 参与这项研究的联系和答复正在等待中。在……里面 此外，我还在继续收到来自肿瘤的最新信息 关于符合资格要求的新病例的登记。四 到目前为止，已经发现了这类病例，他们参与了这项研究 被追捕。针对这些情况，控制措施被随机选择。 关于儿童癌症队列研究，一份完整的提案 已提交，我们期待这些研究参与者的参与 等待当地机构的内部审查委员会和行政审批。 已经建立了一种机制，通过这种机制可以看到研究参与者 在GCRC门诊设施，或由他们的当地医生。GCRC 护士将在当地或通过邮寄获取血液样本，并将其转发到 弗里德曼博士将与该实验室合作的GCRC实验室 从事DNA提取和RNA短细胞培养准备的工作人员。这个 DNA/RNA随后将储存在蒂莫西·雷贝克博士的实验室中， 基因测试将在他和韦伯博士的实验室进行。 弗里德曼博士已经与雷贝克博士和他的工作人员见过面，并已开始培训 在分子生物学技术方面。她将开发更多的检测方法 Rebbeck博士和Rebbeck博士的实验室中潜在的癌症易感基因 韦伯。每两个月与梅多斯博士举行一次会议，梅多斯博士是 关于这一项目的进展和潜在的困难领域。

This study is now open and to date 38 participants from The Children's Hospital of Philadelphia have been enrolled. Consent forms have been signed. Complete family histories have been obtained from these patients and I anticipate collection of blood samples for genotype testing to commence within the next month. Other cases of second malignancies and appropriate controls have been contacted for participation in the study and response is pending. In addition, I am continuing to receive updated information from the tumor registry regarding new cases who would meet eligibility requirements. Four such cases have been found to date and their participation in the study is being sought. Controls are being randomly chosen for these cases. With respect to the Childhood Cancer Cohort Study, a full proposal has been submitted and we expect participation of these study participants pending IRB and administrative approval at the local institutions. A mechanism has been established whereby the study participants are seen in the GCRC outpatient facility, or by their local physicians. The GCRC nurses will obtain blood samples locally or by mail and forward them to the GCRC laboratory where Dr. Friedman will work with the laboratory staff in DNA extraction and short cell culture preparation for RNA. The DNA/RNA will then be stored in the laboratory of Dr. Timothy Rebbeck and genotype testing will be performed in his and Dr. Weber's laboratories. Dr. Friedman has met with Dr. Rebbeck and his staff and has begun training in molecular biology techniques. She will develop additional assays for potential cancer susceptibility genes in the labs of Drs. Rebbeck and Weber. Bi-monthly meetings have been held with Dr. Meadows, the mentor for this project with respect to progress and potential areas of difficulty.