LOW DOSE TETRACYCLINE THERAPY FOR CHRONIC IDIOPATHIC DIARRHEA IN MACAQUES

低剂量四环素治疗猕猴慢性特发性腹泻

• 批准号: 6277447
• 负责人: JR RUDOLF P BOHM
• 金额: $ 16.99万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6277447
• 关键词: Mammalia; Primates; drug /agent; intestines; model design /development

Chronic diarrhea is the most prevalent clinical disease entity in nonhumanprimate colonies worldwide. Often an etiologic agent is not found. In addition, many animals fail to respond to symptomatic therapy. In the past, the veterinary staff had found that many of these animals responded quickly to low dose tetracycline therapy. A study was undertaken to evaluate clinical response in animals with chronic idiopathic diarrhea using low dose tetracycline therapy. Prior to entrollment on the study, animals had demonstrated diarrhea for no less than 30 consecutive days, had three negative stool samples (flotation, giardia ELISA, cryptosporidial ELISA, sedimentation) and had three negative bacterial rectal swab cultures. Viral culture and antibody testing was performed for simian retrovirus (SRV). A complete blood count (CBC) serum chemistry and colonic endoscopy with biopsy were also performed prior to inclusion on the study. Monitoring during the study period was fulfilled by physical examination, CBC, serum chemistry, bacterial rectal swab culture, fecal exam for parasites, and colonic endoscopy with biopsy. These disagnostics were repeated weekly during dosing, one week after discontinuation of the drug, and two months after discontinuation of the drug. Tetracycline was administered for 30 days once daily at 40 ml regardless of body weight. Eighteen Macaca mulatta from the Tulane Regional Primate Research Center breeding colony were used for the study. Animals ranged in age from 1.67 to 19 years. Sixty-seven percent (12/18) of the animals responded to tetracycline therapy by producing normal stools. Days of diarrhea prior to treatment ranged from 30 to 114. Relapse rate after therapy was withdrawn was 42%. Rate of response to therapy or relapse rate were not correlated with age or length of time of diarrhea prior to treatment. Physical examination revealed an increase in mean body weight and normalization of hydration status during treatment. Histopathology prior to and after tetracycline therapy revealed no significant changes within and comparing responders with non responders. All histo sections were described as normal. Future studies will involve pharmacokinetic evaluation of peripheral and intestinal

慢性腹泻是我国最常见的临床疾病， 非人类灵长类动物的聚居地 通常病原体不是 found. 此外，许多动物对有症状的 疗法 过去，兽医人员发现， 这些动物对低剂量四环素治疗反应迅速。 一 进行了一项研究，以评估动物的临床反应， 使用低剂量四环素治疗慢性特发性腹泻。 入组研究前，动物出现腹泻 连续不少于30天，有三个阴性粪便样本 （浮选、贾第虫ELISA、隐孢子虫ELISA、沉降）和 有三个直肠拭子细菌培养阴性 病毒培养和 进行猴逆转录病毒（SRV）的抗体测试。 一 全血细胞计数（CBC）血清化学和结肠内窥镜检查， 在纳入研究之前还进行了活检。 研究期间的监测由物理 检查，CBC，血清化学，细菌直肠拭子培养， 粪便寄生虫检查结肠内窥镜检查和活检 这些 给药期间每周重复诊断，给药后一周 停药后两个月， 流毒 四环素给药30天，每日一次，40 无论体重如何，ml。 十八只杜兰号上的猕猴 区域灵长类动物研究中心的繁殖群被用于 study. 动物年龄范围为1.67 - 19岁。 六十七 %（12/18）的动物对四环素治疗有反应， 产生正常的粪便。 治疗前腹泻天数范围 从30到114 停药后复发率为42%。 对治疗的反应率或复发率与 治疗前腹泻的年龄或时间长度。 物理 检查显示平均体重增加， 治疗期间的水合状态。 之前和之后的组织学 四环素治疗后， 比较有反应者和无反应者。 所有组织切片均为 描述为正常。 未来的研究将涉及药代动力学 外周和肠道评价