CORE--BIOMOLECULAR FACILITY

核心--生物分子设施

• 批准号: 6100402
• 负责人: MINORU SATOH
• 金额: --
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-15 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6100402
• 关键词: animal colony; biomedical facility; computer center; digital imaging; flow cytometry; image processing; information systems; laboratory mouse; spectrophosphorimetry

The Biomolecular Core has continued to evolve by providing new services when required, while eliminating other services that became unnecessary. The newest function added during the current year is a Biomolecular Imaging Facility consisting of a Molecular Dynamic Phosphorimager and a Digital Microscopy Facility. The latter is still under development, and will provide MAMDC investigators with state-of-the-art facilities for microscopic image acquisition, analysis, and manipulation. Other functions of the Biomolecular Core include 1) an autoimmune serum blank, cell blank, and clinical databases, 2) a flow cytometry facility, and 3) an inbred mouse colony. Additional functions, formerly par of the Biomolecular Core, are now free-standing and no-longer require support. These include the DNA Sequencing Facility, which was organized under the auspices of the MAMDC and the Program in Molecular Biology, and is now self-supporting. The protein purification and characterization facility also has been eliminated because many of the techniques are well established in individual investigators' laboratories or have been incorporated into the peptide synthesis/analysis facility directed by Dr. David Klapper (see Progress Report). Finally, maintenance of the Center's computing facilities will be taken over by the Numerical Sciences Core (NUSCOR). The purpose of the Biomolecular Core is to provide functions that are required by a large number of investigators but which can not easily be duplicated within each of the individual laboratories. This includes the acquisition, upgrading, and maintenance of costly equipment utilized by several of the individual laboratories involved in biomedical research. Centralizing these facilities results in an economy of scale, allowing access to new technology at a substantially discounted cost to the investigator.

生物分子核心通过提供新的服务而继续进化 当需要时，同时取消其他不必要的服务。 今年增加的最新功能是生物分子 成像设备由分子动态磷光成像仪和 数字显微镜设备。后者仍在开发中，而且 将为MAMDC调查人员提供最先进的设施 显微图像的获取、分析和处理。其他功能 生物分子核心包括1)自身免疫血清空白，细胞空白， 和临床数据库，2)流式细胞仪设备，3)近交系 老鼠聚居地。附加功能，以前是生物分子核心的PAR， 现在是独立的，不再需要支持。其中包括DNA 测序设施，这是在MAMDC的主持下组织的 和分子生物学的项目，现在是自给自足的。这个 蛋白质纯化和鉴定设施也已经 被淘汰是因为许多技术在 个别调查人员的实验室或已纳入 大卫·克拉珀博士指导的多肽合成/分析设备(见 进度报告)。最后，维护中心的计算能力 设施将由数字科学核心(NUSCOR)接管。这个 生物分子核心的目的是提供所需的功能 由大量调查人员提供，但不容易复制 在每个单独的实验室里。这包括收购， 升级和维护几个国家使用的昂贵设备 参与生物医学研究的各个实验室。集中化 这些设施产生了规模经济，允许获得新的 技术，对调查者来说，成本大大打折。