OXIDATION OF PYROLYTIC CARBON
OXIDATION OF PYROLYTIC CARBON
批准号:
6119947
负责人:
G PICCIOLO
金额:
$0.42万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-07-01 至 1999-09-27
中文摘要
本研究旨在确定人类单核/巨噬细胞
热解碳(PYC)的培养会使表面氧化。氧化
通过多种方法获得热解碳,以及
表面分析方法被用来比较表面因此
在细胞黏附研究后观察到的那些
已执行。SIMS已被证明是最有用的技术
对准备好的表面进行表征,并确定如何
在清除粘附物方面取得了成功的各种清洁策略
蛋白质和细胞,这是后续样品分析所必需的。
英文摘要
This study aims to determine whether human monocytes/macrophages
culturedon pyrolytic carbon (PYC) can oxidize the surface. Oxidation
of the pyrolitic carbon is achieved by a number of methods, and
surface analytical methods are employed to compare the surfaces so
obtained to those observed after cell adhesion studies have been
performed. SIMS has proven to be the most useful technique to
characterize the prepared surface as well as to determine how
successful various cleaning strategies are in removing adherent
proteins and cells, which is necessary for subsequent sample analysis.
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